RT Journal Article
T1 The cobas® HCV GT is a new tool that accurately identifies Hepatitis C virus genotypes for clinical practice
A1 Fernández-Caballero, J A
A1 Alvarez, M
A1 Chueca, N
A1 Pérez, A B
A1 García, F
K1 Secuencia de bases
K1 Genotipo
K1 Humanos
K1 Patología molecular
K1 Filogenia
K1 Análisis de secuencia de ADN
AB OBJECTIVE:We aimed to evaluate the correct assignment of HCV genotype/subtypes 1a and 1b by cobas® HCV genotyping (GT) assay (Roche Molecular Diagnostics) compared with nonstructural protein 5B (NS5B) sequencing.PATIENTS AND METHODS:Clinical samples from 153 patients submitted for HCV genotyping were studied. After genotyping with the cobas® HCV GT, sequencing of a 387 bp fragment in the NS5B gene and phylogenetic analysis was employed to compare genotyping results. Major discrepancies were defined as differences in the assigned genotype by cobas® HCV GT and NS5B sequencing (including genotype 1 subtypes 1a and 1b misclassification).RESULTS:Overall agreement between the cobas® HCV GT and NS5B sequencing was 98%; all the 1a, 1b, 2, 3 and 4 genotypes identified by cobas® HCV GT were concordant with NS5B sequencing. Three samples tested "indetermined" by cobas® HCV GT assay and were genotyped as 1a, 3a, and 4d by NS5B sequencing.CONCLUSSION:These results indicate that the cobas® HCV GT assay correctly identifies HCV genotypes, and points out the importance of additional methods based on DNA sequencing for resolving indeterminate results.
PB Public Library of Science
YR 2017
FD 2017-04-14
LK http://hdl.handle.net/10668/2642
UL http://hdl.handle.net/10668/2642
LA en
NO Fernández-Caballero JA, Alvarez M, Chueca N, Pérez AB, García F. The cobas® HCV GT is a new tool that accurately identifies Hepatitis C virus genotypes for clinical practice. PLoS One. 2017 Apr 14;12(4):e0175564.
DS RISalud
RD Apr 12, 2025