%0 Journal Article
%A Fernández-Caballero, J A
%A Alvarez, M
%A Chueca, N
%A Pérez, A B
%A García, F
%T The cobas® HCV GT is a new tool that accurately identifies Hepatitis C virus genotypes for clinical practice
%D 2017
%U http://hdl.handle.net/10668/2642
%X OBJECTIVE:We aimed to evaluate the correct assignment of HCV genotype/subtypes 1a and 1b by cobas® HCV genotyping (GT) assay (Roche Molecular Diagnostics) compared with nonstructural protein 5B (NS5B) sequencing.PATIENTS AND METHODS:Clinical samples from 153 patients submitted for HCV genotyping were studied. After genotyping with the cobas® HCV GT, sequencing of a 387 bp fragment in the NS5B gene and phylogenetic analysis was employed to compare genotyping results. Major discrepancies were defined as differences in the assigned genotype by cobas® HCV GT and NS5B sequencing (including genotype 1 subtypes 1a and 1b misclassification).RESULTS:Overall agreement between the cobas® HCV GT and NS5B sequencing was 98%; all the 1a, 1b, 2, 3 and 4 genotypes identified by cobas® HCV GT were concordant with NS5B sequencing. Three samples tested "indetermined" by cobas® HCV GT assay and were genotyped as 1a, 3a, and 4d by NS5B sequencing.CONCLUSSION:These results indicate that the cobas® HCV GT assay correctly identifies HCV genotypes, and points out the importance of additional methods based on DNA sequencing for resolving indeterminate results.
%K Secuencia de bases
%K Genotipo
%K Humanos
%K Patología molecular
%K Filogenia
%K Análisis de secuencia de ADN
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