RT Journal Article
T1 Bifidobacterium longum subsp. infantis CECT 7210 Reduces Inflammatory Cytokine Secretion in Caco-2 Cells Cultured in the Presence of Escherichia coli CECT 515.
A1 Alvarez-Mercado, Ana I
A1 Plaza-Diaz, Julio
A1 de Almagro, M Cristina
A1 Gil, Angel
A1 Moreno-Muñoz, Jose Antonio
A1 Fontana, Luis
K1 B. infantis IM-1®
K1 Bifidobacterium longum subsp. infantis CECT 7210
K1 Caco-2 cells
K1 Escherichia coli
K1 co-cultures
K1 cytokines
K1 dendritic cells
K1 probiotics
AB Previous works have described the activity of Bifidobacterium longum subsp. infantis CECT 7210 (also commercially named B. infantis IM-1®) against rotavirus in mice and intestinal pathogens in piglets, as well as its diarrhea-reducing effect on healthy term infants. In the present work, we focused on the intestinal immunomodulatory effects of B. infantis IM-1® and for this purpose we used the epithelial cell line isolated from colorectal adenocarcinoma Caco-2 and a co-culture system of human dendritic cells (DCs) from peripheral blood together with Caco-2 cells. Single Caco-2 cultures and Caco-2: DC co-cultures were incubated with B. infantis IM-1® or its supernatant either in the presence or absence of Escherichia coli CECT 515. The B. infantis IM-1® supernatant exerted a protective effect against the cytotoxicity caused by Escherichia coli CECT 515 on single cultures of Caco-2 cells as viability reached the values of untreated cells. B. infantis IM-1® and its supernatant also decreased the secretion of pro-inflammatory cytokines by Caco-2 cells and the co-cultures incubated in the presence of E. coli CECT 515, with the response being more modest in the latter, which suggests that DCs modulate the activity of Caco-2 cells. Overall, the results obtained point to the immunomodulatory activity of this probiotic strain, which might underlie its previously reported beneficial effects.
PB MDPI AG
YR 2022
FD 2022-09-06
LK http://hdl.handle.net/10668/21196
UL http://hdl.handle.net/10668/21196
LA en
NO Álvarez-Mercado AI, Plaza-Díaz J, de Almagro MC, Gil Á, Moreno-Muñoz JA, Fontana L. Bifidobacterium longum subsp. infantis CECT 7210 Reduces Inflammatory Cytokine Secretion in Caco-2 Cells Cultured in the Presence of Escherichia coli CECT 515. Int J Mol Sci. 2022 Sep 16;23(18):10813.
DS RISalud
RD Apr 9, 2025