RT Journal Article
T1 In vitro efficacy of daptomycin and teicoplanin combined with ethanol, clarithromycin or gentamicin as catheter lock solutions.
A1 Parra, Diego
A1 Peña-Monje, Alejandro
A1 Coronado-Álvarez, Nieves María
A1 Hernández-Quero, José
A1 Parra-Ruiz, Jorge
K1 Central line associated bloodstream infections
K1 Antibiotic lock therapy
K1 Biofilm
K1 Antibacterianos
K1 Catéteres
K1 Claritromicina
K1 Daptomicina
K1 Quimioterapia combinada
K1 Etanol
K1 Biofilmes
K1 Gentamicinas
K1 Humanos
K1 Técnicas In vitro
K1 Resistencia a la meticilina
K1 Pruebas de sensibilidad microbiana
K1 Teicoplanina
K1 Vancomicina
AB BACKGROUNDDespite widespread use, optimum choice of antimicrobial agents, concentrations, combinations and exposure times have not been determined for antibiotic lock technique (ALT). Our objective was to evaluate the efficacy of different antibiotic combinations using an in vitro model of catheter-related infection. Daptomycin (DAP) 5 mg/mL, teicoplanin (TEC) 5 mg/mL, both alone and combined with gentamicin (GM) 2.5 mg/mL, clarythromycin (CLA) 5 mg/mL or ethanol 35 % were evaluated against four clinical strains of methicillin resistant coagulase negative staphylococci. Lock solutions were renewed every 24 h.RESULTSAfter 72 h catheters were reincubated with culture media to investigate bacterial regrowth. All antibiotic combinations resulted in significant reductions (p < 0.05) of Log(10) cfu/mL at 72 h for both organisms compared with controls. DAP resulted in significant reductions of Log(10) for all organism versus TEC (p = 0.001). Only DAP reached the limit of detection at 72 h, however did not prevent regrowth after 24 h of ALT removal. DAP + Ethanol and TEC + ethanol eradicated biofilm at 72 h, but only DAP + ethanol (against all strains) and DAP + CLA (against two strains) prevented regrowth at 24 h after ALT removal.CONCLUSIONSBased on these data, ALT with DAP + ethanol and DAP + CLA should be explored in clinical trials.
PB BioMed Central
YR 2015
FD 2015-10-30
LK http://hdl.handle.net/10668/2479
UL http://hdl.handle.net/10668/2479
LA en
NO Parra D, Peña-Monje A, Coronado-Álvarez NM, Hernández-Quero J, Parra-Ruiz J. In vitro efficacy of daptomycin and teicoplanin combined with ethanol, clarithromycin or gentamicin as catheter lock solutions. BMC Microbiol. 2015.; 15:245
NO Journal Article;
DS RISalud
RD Apr 11, 2025