RT Journal Article
T1 Late rDNA Condensation Ensures Timely Cdc14 Release and Coordination of Mitotic Exit Signaling with Nucleolar Segregation.
A1 de Los Santos-Velázquez, Ana Isabel
A1 de Oya, Inés G
A1 Manzano-López, Javier
A1 Monje-Casas, Fernando
K1 Cdc14
K1 FEAR
K1 MEN
K1 condensin
K1 mitotic exit
K1 nucleolus
K1 snoRNPs
AB The nucleolus plays a pivotal role in multiple key cellular processes. An illustrative example is the regulation of mitotic exit in Saccharomyces cerevisiae through the nucleolar sequestration of the Cdc14 phosphatase. The peculiar structure of the nucleolus, however, has also its drawbacks. The repetitive nature of the rDNA gives rise to cohesion-independent linkages whose resolution in budding yeast requires the Cdc14-dependent inhibition of rRNA transcription, which facilitates condensin accessibility to this locus. Thus, the rDNA condenses and segregates later than most other yeast genomic regions. Here, we show that defective function of a small nucleolar ribonucleoprotein particle (snoRNP) assembly factor facilitates condensin accessibility to the rDNA and induces nucleolar hyper-condensation. Interestingly, this increased compaction of the nucleolus interferes with the proper release of Cdc14 from this organelle. This observation provides an explanation for the delayed rDNA condensation in budding yeast, which is necessary to efficiently coordinate timely Cdc14 release and mitotic exit with nucleolar compaction and segregation.
YR 2017
FD 2017-10-19
LK http://hdl.handle.net/10668/11711
UL http://hdl.handle.net/10668/11711
LA en
DS RISalud
RD Apr 19, 2025