RT Journal Article
T1 Functional characterization of E- and P-cadherin in invasive breast cancer cells
A1 Sarrió, David
A1 Palacios, José
A1 Hergueta-Redondo, Marta
A1 Gómez-López, Gonzalo
A1 Cano, Amparo
A1 Moreno-Bueno, Gema
K1 Cadherinas
K1 Línea Celular Tumoral
K1 Movimiento Celular
K1 Regulación Neoplásica de la Expresión Génica
K1 Invasividad Neoplásica
K1 Transcripción Genética
K1 Transfección
K1 Neoplasias de la Mama
AB BACKGROUNDAlterations in the cadherin-catenin adhesion complexes are involved in tumor initiation, progression and metastasis. However, the functional implication of distinct cadherin types in breast cancer biology is still poorly understood.METHODSTo compare the functional role of E-cadherin and P-cadherin in invasive breast cancer, we stably transfected these molecules into the MDA-MB-231 cell line, and investigated their effects on motility, invasion and gene expression regulation.RESULTSExpression of either E- and P-cadherin significantly increased cell aggregation and induced a switch from fibroblastic to epithelial morphology. Although expression of these cadherins did not completely reverse the mesenchymal phenotype of MDA-MB-231 cells, both E- and P-cadherin decreased fibroblast-like migration and invasion through extracellular matrix in a similar way. Moreover, microarray gene expression analysis of MDA-MB-231 cells after expression of E- and P-cadherins revealed that these molecules can activate signaling pathways leading to significant changes in gene expression. Although the expression patterns induced by E- and P-cadherin showed more similarities than differences, 40 genes were differentially modified by the expression of either cadherin type.CONCLUSIONE- and P-cadherin have similar functional consequences on the phenotype and invasive behavior of MDA-MB-231 cells. Moreover, we demonstrate for the first time that these cadherins can induce both common and specific gene expression programs on invasive breast cancer cells. Importantly, these identified genes are potential targets for future studies on the functional consequences of altered cadherin expression in human breast cancer.
PB BIOMED CENTRAL LTD
YR 2009
FD 2009-03-03
LK http://hdl.handle.net/10668/1794
UL http://hdl.handle.net/10668/1794
LA en
NO Sarrió D, Palacios J, Hergueta-Redondo M, Gómez-López G, Cano A, Moreno-Bueno G. Functional characterization of E- and P-cadherin in invasive breast cancer cells. BMC Cancer. 2009; 9:74
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 6, 2025