RT Journal Article
T1 Bioavailability and biotransformation of linolenic acid from basil seed oil as a novel source of omega-3 fatty acids tested on a rat experimental model.
A1 Martinez, Rosario
A1 Mesas, Cristina
A1 Guzman, Ana
A1 Galisteo, Milagros
A1 Lopez-Jurado, Maria
A1 Prados, Jose
A1 Melguizo, Consolacion
A1 Bermudez, Francisco
A1 Porres, Jesus M
K1 Animals
K1 Arachidonic Acid
K1 Biological Availability
K1 Biotransformation
K1 Eicosapentaenoic Acid
AB Basil is an aromatic herb with a high concentration of bioactive compounds. The oil extracted from its seeds is a good source of α-linolenic acid (ALA) and also provides substantial amounts of linoleic acid (LA). This study aimed to test the bioavailability of the oil derived from basil seeds and its effects on different physiological parameters using 7-15% dietary inclusion levels. Furthermore, the assimilation of LA and ALA and their transformation in long-chain polyunsaturated fatty acids (LC-PUFAs) have been studied. Digestive utilization of total fat from basil seed oil (BSO) was high and similar to that of olive oil used as a control. Consumption of BSO resulted in increased LA and ALA levels of the plasma, liver, and erythrocyte membrane. In addition, the transformation of LA to arachidonic acid (ARA) was decreased by the high dietary intake of ALA which redirected the pathway of the Δ-6 desaturase enzyme towards the transformation of ALA into eicosapentaenoic acid (EPA). No alterations of hematological and plasma biochemical parameters were found for the 7 and 10% dietary inclusion levels of BSO, whereas a decrease in the platelet count and an increase in total- and HDL-cholesterol as well as plasma alkaline phosphatase (ALP) were found for a 15% BSO dose. In conclusion, BSO is a good source of ALA to be transformed into EPA and decrease the precursor of the pro-inflammatory molecule ARA. This effect on the levels of EPA in different tissues offers potential for its use as a dietary supplement, novel functional food, or a constituent of nutraceutical formulations to treat different pathologies.
PB Royal Society of Chemistry
YR 2022
FD 2022-06-06
LK http://hdl.handle.net/10668/19644
UL http://hdl.handle.net/10668/19644
LA en
NO Martínez R, Mesas C, Guzmán A, Galisteo M, López-Jurado M, Prados J, et al. Bioavailability and biotransformation of linolenic acid from basil seed oil as a novel source of omega-3 fatty acids tested on a rat experimental model. Food Funct. 2022 Jul 18;13(14):7614-7628.
NO The authors want to thank Susana Ibáñez from the Analytical Unit of Scientific Instrumentation Centre (CIC, UGR) for its excellent technical assistance and Antonio Murillo Cancho from the University of Almería and CELLBITEC S.L. This research was funded by the Spanish Ministry of Science, Innovation, and Universities as well as the European Union through projects PTQ-17-09172, RTC-2017-6540-1, RTI2018-100934-B-I00, and RTC2019-006870-1, and the FEDER program. In addition, this work was supported by funds from research groups AGR145, CTS164, and CTS-107 (Andalusian Government).
DS RISalud
RD Apr 7, 2025