RT Journal Article
T1 Oral saliva swab reverse transcription PCR for Covid-19 in the paediatric population.
A1 Moraleda, Cinta
A1 Domínguez-Rodríguez, Sara
A1 Mesa, Juan Miguel
A1 García-Sánchez, Paula
A1 de la Serna, María
A1 Alonso-Cadenas, José Antonio
A1 Bermejo, Amanda
A1 Sabrido, Gema
A1 Martínez-Campos, Leticia
A1 González-Posada, Aránzazu Flavia
A1 Illán-Ramos, Marta
A1 Cobos-Carrascosa, Elena
A1 Ballesteros, Álvaro
A1 Galán, Juan Carlos
A1 Llorente, Francisco
A1 Aguilera-Alonso, David
A1 Jiménez, Ana Belén
A1 Catalán, Pilar
A1 Dahdouh, Elias
A1 Navarro, Ignacio
A1 Fernández-Garoz, Bárbara
A1 Mendoza, Pablo
A1 Pérez-Jorge, Concepción
A1 Cabezas-Fernández, Teresa
A1 Blázquez-Gamero, Daniel
A1 Rivas, Gonzalo
A1 Gonzalez-Donapetry, Patricia
A1 Sáez, Elena
A1 Fernández-Pinero, Jovita
A1 Lucas-Fernández, Jesús
A1 Pérez-Ramírez, Elisa
A1 Merino, Paloma
A1 Miragaya, Sandra
A1 Lorente, Jorge
A1 Iglesias, Irene
A1 Tagarro, Alfredo
A1 EPICO working group, 
K1 Covid-19
K1 Infectious Disease Medicine
K1 Microbiology
K1 Molecular Biology
K1 Virology
AB To evaluate the performance of oral saliva swab (OSS) reverse transcription PCR (RT-PCR) compared with RT-PCR and antigen rapid diagnostic test (Ag-RDT) on nasopharyngeal swabs (NPS) for SARS-CoV-2 in children. Cross-sectional multicentre diagnostic study. Study nested in a prospective, observational cohort (EPICO-AEP) performed between February and March 2021 including 10 hospitals in Spain. Children from 0 to 18 years with symptoms compatible with Covid-19 of ≤5 days of duration were included. Two NPS samples (Ag-RDT and RT-PCR) and one OSS sample for RT-PCR were collected. Performance of Ag-RDT and RT-PCR on NPS and RT-PCR on OSS sample for SARS-CoV-2. 1174 children were included, aged 3.8 years (IQR 1.7-9.0); 73/1174 (6.2%) patients tested positive by at least one of the techniques. Sensitivity and specificity of OSS RT-PCR were 72.1% (95% CI 59.7 to 81.9) and 99.6% (95% CI 99 to 99.9), respectively, versus 61.8% (95% CI 49.1 to 73) and 99.9% (95% CI 99.4 to 100) for the Ag-RDT. Kappa index was 0.79 (95% CI 0.72 to 0.88) for OSS RT-PCR and 0.74 (95% CI 0.65 to 0.84) for Ag-RDT versus NPS RT-PCR. RT-PCR on the OSS sample is an accurate option for SARS-CoV-2 testing in children. A less intrusive technique for younger patients, who usually are tested frequently, might increase the number of patients tested.
YR 2022
FD 2022-06-10
LK http://hdl.handle.net/10668/20131
UL http://hdl.handle.net/10668/20131
LA en
DS RISalud
RD Apr 9, 2025