RT Journal Article
T1 Sweat as a clinical sample: what is done and what should be done.
A1 Luque de Castro, Maria Dolores
K1 sweat metabolomics
K1 sweat normalization
K1 sweat sample preparation
K1 sweat samplers
K1 sweat xenometabolomics
AB Sweat is known for being a clear, hypotonicbiofluid produced by eccrine and procrineglands located in the epidermis, with aslightly acidic pH (between 4.0 and 6.8), andcomposed mainly by water (99%), contain-ing the so-called electrolytes (e.g., sodium,chloride and potassium), urea, pyruvate andlactate; but also proteins, peptides, amines,amino acids and metal ions in smaller con-centrations, together with inhibitors, anti-gens, antibodies and a variety of xenobioticssuch as drugs, cosmetics and ethanol [1]. Theclinical importance of sweat has traditionallybeen limited to the determination of chlo-ride for the diagnosis of cystic fibrosis (CF)and incipient determination of drugs [1]. Thepresent spread of ‘omics’ disciplines, and par-ticularly of metabolomics as the youngestof the big ‘omics,’ has open a fan of possi-bilities to the use of sweat as clinical sample.Except for the case of some high molecularweight proteins, which reach sweat by dif-ferent intracellular storages in particularsituations [2,3], most sweat components aresmall molecules resulting from metabolicpathways; therefore, their study pertains tothe metabolomics field, the omics of smallmolecules typically (<1000 Da or <1500 Da).
PB Taylor & Francis
YR 2015
FD 2015-10-21
LK http://hdl.handle.net/10668/9657
UL http://hdl.handle.net/10668/9657
LA en
NO Luque de Castro MD. Sweat as a clinical sample: what is done and what should be done. Bioanalysis. 2016;8(2):85-8.
DS RISalud
RD Apr 20, 2025