RT Journal Article
T1 Reduction of mRNA export unmasks different tissue sensitivities to low mRNA levels during Caenorhabditis elegans development.
A1 Zheleva, Angelina
A1 Gomez-Orte, Eva
A1 Saenz-Narciso, Beatriz
A1 Ezcurra, Begoña
A1 Kassahun, Henok
A1 de-Toro, Maria
A1 Miranda-Vizuete, Antonio
A1 Schnabel, Ralf
A1 Nilsen, Hilde
A1 Cabello, Juan
K1 Amino Acid Sequence
K1 Caenorhabditis elegans Proteins
K1 Nucleocytoplasmic Transport Proteins
K1 RNA-Binding Proteins
AB Animal development requires the execution of specific transcriptional programs in different sets of cells to build tissues and functional organs. Transcripts are exported from the nucleus to the cytoplasm where they are translated into proteins that, ultimately, carry out the cellular functions. Here we show that in Caenorhabditis elegans, reduction of mRNA export strongly affects epithelial morphogenesis and germline proliferation while other tissues remain relatively unaffected. Epithelialization and gamete formation demand a large number of transcripts in the cytoplasm for the duration of these processes. In addition, our findings highlight the existence of a regulatory feedback mechanism that activates gene expression in response to low levels of cytoplasmic mRNA. We expand the genetic characterization of nuclear export factor NXF-1 to other members of the mRNA export pathway to model mRNA export and recycling of NXF-1 back to the nucleus. Our model explains how mutations in genes involved in general processes, such as mRNA export, may result in tissue-specific developmental phenotypes.
PB Public Library of Science
YR 2019
FD 2019-09-16
LK http://hdl.handle.net/10668/14510
UL http://hdl.handle.net/10668/14510
LA en
NO Zheleva A, Gómez-Orte E, Sáenz-Narciso B, Ezcurra B, Kassahun H, de Toro M, et al.  Reduction of mRNA export unmasks different tissue sensitivities to low mRNA levels during Caenorhabditis elegans development. PLoS Genet. 2019 Sep 16;15(9):e1008338.
DS RISalud
RD Jul 6, 2025