TY  - JOUR
AU  - Chato-Astrain, Jesús
AU  - Sánchez-Porras, David
AU  - García-García, Óscar Darío
AU  - Vairo, Claudia
AU  - Villar-Vidal, María
AU  - Villullas, Silvia
AU  - Sánchez-Montesinos, Indalecio
AU  - Campos, Fernando
AU  - Garzón, Ingrid
AU  - Alaminos, Miguel
PY  - 2021
DO  - 10.3390/biomedicines9111634
UR  - http://hdl.handle.net/10668/3494
T2  - Biomedicines
AB  - Human skin keratinocyte primary cultures can be established from skin biopsies with culture media containing epithelial growth factor (EGF). Although current methods are efficient, optimization is required to accelerate the procedure and obtain these...
LA  - en
PB  - MDPI
KW  - EGF
KW  - Nanoparticles
KW  - NLC
KW  - Keratinocytes
KW  - Tissue engineering
KW  - Cell culture
KW  - Epidermal growth factor
KW  - Factor de crecimiento epidérmico
KW  - Nanopartículas
KW  - Queratinocitos
KW  - Ingeniería de tejidos
KW  - Técnicas de cultivo de célula
KW  - Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
KW  - Medical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Gastrointestinal Hormones::Epidermal Growth Factor
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Containment of Biohazards
KW  - Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Feeder Cells
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Cell Culture Techniques::Primary Cell Culture
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Culture Techniques::Cell Engineering::Tissue Engineering
KW  - Medical Subject Headings::Chemicals and Drugs::Lipids
KW  - Medical Subject Headings::Anatomy::Cells::Epithelial Cells::Keratinocytes
KW  - Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line
KW  - Medical Subject Headings::Anatomy::Cells::Epithelial Cells
KW  - Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation
KW  - Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression
KW  - Medical Subject Headings::Anatomy::Cells::Cellular Structures::Cell Membrane::Cell Membrane Structures::Intercellular Junctions
KW  - Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Specialty Uses of Chemicals::Laboratory Chemicals::Culture Media
KW  - Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::Intermediate Filament Proteins::Keratins
KW  - Medical Subject Headings::Phenomena and Processes::Digestive System and Oral Physiological Phenomena::Digestive System Physiological Phenomena::Digestive System Processes::Digestion
KW  - Medical Subject Headings::Anatomy::Sense Organs::Eye::Anterior Eye Segment::Cornea
TI  - Improvement of Cell Culture Methods for the Successful Generation of Human Keratinocyte Primary Cell Cultures Using EGF-Loaded Nanostructured Lipid Carriers
TY  - review article
ER  -