RT Journal Article
T1 Carbapenemase-producing Escherichia coli is becoming more prevalent in Spain mainly because of the polyclonal dissemination of OXA-48
A1 Ortega, Adriana
A1 Saez, David
A1 Bautista, Veronica
A1 Fernandez-Romero, Sara
A1 Lara, Noelia
A1 Aracil, Belen
A1 Perez-Vazquez, Maria
A1 Campos, Jose
A1 Oteo, Jesus
A1 Spanish Collaborating Grp, 
K1 Sequence type 131
K1 Klebsiella-pneumoniae
K1 Beta-lactamase
K1 Molecular epidemiology
K1 Resistant enterobacteriaceae
K1 Emergence
K1 Spread
K1 Hospitals
K1 Susceptibility
K1 Virulence
AB The objective of this study was to analyse the microbiological traits and the population structure of carbapenemase-producing (CP) Escherichia coli isolates collected in Spain between 2012 and 2014.Two-hundred-and-thirty-nine E. coli isolates non-susceptible to carbapenems were studied. The carbapenemase genes and the phylogenetic groups were characterized using PCR. MLST was carried out using the typing schemes of the University of Warwick and the Institut Pasteur. The diversity of the population structure was estimated by calculating a simple diversity index (SDI).One-hundred-and-twenty-one isolates (50.6%) produced carbapenemases, of which 87 (71.9%) were OXA-48, 27 (22.3%) were VIM-1, 4 (3.3%) were KPC-2, 2 (1.7%) were NDM and 1 (0.8%) was IMP-22; 4 isolates were collected in 2012, 40 in 2013 and 77 in 2014. Ertapenem was more sensitive than imipenem or meropenem for screening for OXA-48-producing E. coli. Using the Warwick typing scheme, 59 different STs were identified, the most prevalent being ST131 (16.5%). The population diversity was higher among VIM-1-producing isolates (SDIaEuroS=aEuroS81.5%) than among OXA-48-producing isolates (SDIaEuroS=aEuroS44.8%). The Pasteur scheme had a higher discrimination capability (SDIaEuroS=aEuroS55.4%) than the Warwick scheme (SDIaEuroS=aEuroS48.8%).A progressive increase in the prevalence of CP E. coli was observed, mainly due to the dissemination of OXA-48 producers. The most sensitive method for detecting decreased susceptibility of CP E. coli to carbapenems was disc diffusion with ertapenem using the EUCAST screening cut-offs. The spread of CP E. coli was due to a polyclonal population. The Pasteur scheme showed the highest discrimination power. Surveillance is crucial for the early detection of CP E. coli.
PB Oxford univ press
SN 0305-7453
YR 2016
FD 2016-08-01
LK https://hdl.handle.net/10668/24874
UL https://hdl.handle.net/10668/24874
LA en
DS RISalud
RD Apr 12, 2025