RT Journal Article
T1 Pancreatic differentiation of Pdx1-GFP reporter mouse induced pluripotent stem cells.
A1 Porciuncula, Angelo
A1 Kumar, Anujith
A1 Rodriguez, Saray
A1 Atari, Maher
A1 Araña, Miriam
A1 Martin, Franz
A1 Soria, Bernat
A1 Prosper, Felipe
A1 Verfaillie, Catherine
A1 Barajas, Miguel
K1 Differentiation
K1 Induced pluripotent stem cells
K1 Mouse
K1 Pancreas
K1 Pdx1
K1 Reprogramming
AB Efficient induction of defined lineages in pluripotent stem cells constitutes the determinant step for the generation of therapeutically relevant replacement cells to potentially treat a wide range of diseases, including diabetes. Pancreatic differentiation has remained an important challenge in large part because of the need to differentiate uncommitted pluripotent stem cells into highly specialized hormone-secreting cells, which has been shown to require a developmentally informed step-by-step induction procedure. Here, in the framework of using induced pluripotent stem cells (iPSCs) to generate pancreatic cells for pancreatic diseases, we have generated and characterized iPSCs from Pdx1-GFP transgenic mice. The use of a GFP reporter knocked into the endogenous Pdx1 promoter allowed us to monitor pancreatic induction based on the expression of Pdx1, a pancreatic master transcription factor, and to isolate a pure Pdx1-GFP+ population for downstream applications. Differentiated cultures timely expressed markers specific to each stage and end-stage progenies acquired a rather immature beta-cell phenotype, characterized by polyhormonal expression even among cells highly expressing the Pdx1-GFP reporter. Our findings highlight the utility of employing a fluorescent protein reporter under the control of a master developmental gene in order to devise novel differentiation protocols for relevant cell types for degenerative diseases such as pancreatic beta cells for diabetes.
YR 2016
FD 2016-05-12
LK http://hdl.handle.net/10668/10084
UL http://hdl.handle.net/10668/10084
LA en
DS RISalud
RD Apr 10, 2025