%0 Journal Article
%A Porciuncula, Angelo
%A Kumar, Anujith
%A Rodriguez, Saray
%A Atari, Maher
%A Araña, Miriam
%A Martin, Franz
%A Soria, Bernat
%A Prosper, Felipe
%A Verfaillie, Catherine
%A Barajas, Miguel
%T Pancreatic differentiation of Pdx1-GFP reporter mouse induced pluripotent stem cells.
%D 2016
%U http://hdl.handle.net/10668/10084
%X Efficient induction of defined lineages in pluripotent stem cells constitutes the determinant step for the generation of therapeutically relevant replacement cells to potentially treat a wide range of diseases, including diabetes. Pancreatic differentiation has remained an important challenge in large part because of the need to differentiate uncommitted pluripotent stem cells into highly specialized hormone-secreting cells, which has been shown to require a developmentally informed step-by-step induction procedure. Here, in the framework of using induced pluripotent stem cells (iPSCs) to generate pancreatic cells for pancreatic diseases, we have generated and characterized iPSCs from Pdx1-GFP transgenic mice. The use of a GFP reporter knocked into the endogenous Pdx1 promoter allowed us to monitor pancreatic induction based on the expression of Pdx1, a pancreatic master transcription factor, and to isolate a pure Pdx1-GFP+ population for downstream applications. Differentiated cultures timely expressed markers specific to each stage and end-stage progenies acquired a rather immature beta-cell phenotype, characterized by polyhormonal expression even among cells highly expressing the Pdx1-GFP reporter. Our findings highlight the utility of employing a fluorescent protein reporter under the control of a master developmental gene in order to devise novel differentiation protocols for relevant cell types for degenerative diseases such as pancreatic beta cells for diabetes.
%K Differentiation
%K Induced pluripotent stem cells
%K Mouse
%K Pancreas
%K Pdx1
%K Reprogramming
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