RT Journal Article
T1 Dual control of ROS1-mediated active DNA demethylation by DNA damage-binding protein 2 (DDB2).
A1 Cordoba-Cañero, Dolores
A1 Cognat, Valerie
A1 Ariza, Rafael R
A1 Roldan Arjona, Teresa
A1 Molinier, Jean
K1 Arabidopsis thaliana
K1 AGO4
K1 DDB2
K1 DNA methylation
K1 ROS1
K1 Active DNA demethylation
AB By controlling gene expression, DNA methylation contributes to key regulatory processes during plant development. Genomic methylation patterns are dynamic and must be properly maintained and/or re-established upon DNA replication and active removal, and therefore require sophisticated control mechanisms. Here we identify direct interplay between the DNA repair factor DNA damage-binding protein 2 (DDB2) and the ROS1-mediated active DNA demethylation pathway in Arabidopsis thaliana. We show that DDB2 forms a complex with ROS1 and AGO4 and that they act at the ROS1 locus to modulate levels of DNA methylation and therefore ROS1 expression. We found that DDB2 represses enzymatic activity of ROS1. DNA demethylation intermediates generated by ROS1 are processed by the DNA 3'-phosphatase ZDP and the apurinic/apyrimidinic endonuclease APE1L, and we also show that DDB2 interacts with both enzymes and stimulates their activities. Taken together, our results indicate that DDB2 acts as a critical regulator of ROS1-mediated active DNA demethylation.
PB Wiley-Blackwell Publishing
YR 2017
FD 2017-10-17
LK http://hdl.handle.net/10668/11740
UL http://hdl.handle.net/10668/11740
LA en
NO Córdoba-Cañero D, Cognat V, Ariza RR, Roldán Arjona T, Molinier J. Dual control of ROS1-mediated active DNA demethylation by DNA damage-binding protein 2 (DDB2). Plant J. 2017 Dec;92(6):1170-1181
NO This work was supported by the Spanish Ministry of Science andInnovation and the European Regional Development Fund undergrant BFU2016-80728-P (to TRA) and by the Junta de Andalucíaand the European Regional Development Fund under grant P11-CVI-7576 (to TRA). We thank members of our laboratories for helpful discussions and advic
DS RISalud
RD Apr 11, 2025