RT Journal Article
T1 Rpd3L Contributes to the DNA Damage Sensitivity of Saccharomyces cerevisiae Checkpoint Mutants.
A1 Gómez-González, Belén
A1 Patel, Harshil
A1 Early, Anne
A1 Diffley, John F X
K1 DNA damage checkpoint
K1 Rpd3L
K1 histone deacetylase
K1 replication
K1 replication fork arrest
AB DNA replication forks that are stalled by DNA damage activate an S-phase checkpoint that prevents irreversible fork arrest and cell death. The increased cell death caused by DNA damage in budding yeast cells lacking the Rad53 checkpoint protein kinase is partially suppressed by deletion of the EXO1 gene. Using a whole-genome sequencing approach, we identified two additional genes, RXT2 and RPH1, whose mutation can also partially suppress this DNA damage sensitivity. We provide evidence that RXT2 and RPH1 act in a common pathway, which is distinct from the EXO1 pathway. Analysis of additional mutants indicates that suppression works through the loss of the Rpd3L histone deacetylase complex. Our results suggest that the loss or absence of histone acetylation, perhaps at stalled forks, may contribute to cell death in the absence of a functional checkpoint.
YR 2018
FD 2018-12-17
LK http://hdl.handle.net/10668/13319
UL http://hdl.handle.net/10668/13319
LA en
DS RISalud
RD Apr 17, 2025