RT Journal Article
T1 Acute oxygen sensing in heme oxygenase-2 null mice.
A1 Ortega-Sáenz, Patricia
A1 Pascual, Alberto
A1 Gómez-Díaz, Raquel
A1 López-Barneo, José
K1 Animales
K1 Animales Recién Nacidos
K1 Calcio
K1 Cuerpo Carotídeo
K1 Catecolaminas
K1 Células Cromafines
K1 Ciclofilinas
K1 Expresión Génica
K1 Hemo Oxigenasa (Deciclizadora)
K1 Hemo-Oxigenasa 1
K1 Subunidades alfa de los Canales de Potasio de Gran Conductancia Activados por Calcio
K1 Canales de Potasio de Gran Conductancia Activados por el Calcio
K1 Ratones
K1 Ratones Consanguíneos C57BL
K1 Ratones Noqueados
K1 Tamaño de los Órganos
K1 Oxígeno
K1 Presión Parcial
K1 Técnicas de Placa-Clamp
K1 Péptidos
K1 Bloqueadores de los Canales de Potasio
K1 Ratas
K1 Médula Suprarrenal
AB Hemeoxygenase-2 (HO-2) is an antioxidant enzyme that can modulate recombinant maxi-K(+) channels and has been proposed to be the acute O(2) sensor in the carotid body (CB). We have tested the physiological contribution of this enzyme to O(2) sensing using HO-2 null mice. HO-2 deficiency leads to a CB phenotype characterized by organ growth and alteration in the expression of stress-dependent genes, including the maxi-K(+) channel alpha-subunit. However, sensitivity to hypoxia of CB is remarkably similar in HO-2 null animals and their control littermates. Moreover, the response to hypoxia in mouse and rat CB cells was maintained after blockade of maxi-K(+) channels with iberiotoxin. Hypoxia responsiveness of the adrenal medulla (AM) (another acutely responding O(2)-sensitive organ) was also unaltered by HO-2 deficiency. Our data suggest that redox disregulation resulting from HO-2 deficiency affects maxi-K(+) channel gene expression but it does not alter the intrinsic O(2) sensitivity of CB or AM cells. Therefore, HO-2 is not a universally used acute O(2) sensor.
PB Rockefeller University Press
SN 0022-1295
YR 2006
FD 2006-10
LK http://hdl.handle.net/10668/1703
UL http://hdl.handle.net/10668/1703
LA en
NO Ortega-Sáenz P, Pascual A, Gómez-Díaz R, López-Barneo J. Acute oxygen sensing in heme oxygenase-2 null mice. J Gen Physiol. 2006; 128(4):405-1
NO Journal Article; Research Support, Non-U.S. Gov't;. A Pascual is an investigator of the “Ramón y Cajal” program. J. López-Barneo received the “Ayuda a la Investigación 2000” of the Juan March Foundation
DS RISalud
RD Apr 8, 2025