RT Journal Article
T1 Intestinal Anti-inflammatory Effects of Outer Membrane Vesicles from Escherichia coli Nissle 1917 in DSS-Experimental Colitis in Mice.
A1 Fabrega, Maria-Jose
A1 Rodriguez-Nogales, Alba
A1 Garrido-Mesa, Jose
A1 Algieri, Francesca
A1 Badia, Josefa
A1 Gimenez, Rosa
A1 Galvez, Julio
A1 Baldoma, Laura
K1 DSS-experimental colitis
K1 Escherichia coli Nissle 1917
K1 immune modulation
K1 mouse model
K1 outer membrane vesicles
K1 probiotic
AB Escherichia coli Nissle 1917 (EcN) is a probiotic strain with proven efficacy in inducing and maintaining remission of ulcerative colitis. However, the microbial factors that mediate these beneficial effects are not fully known. Gram-negative bacteria release outer membrane vesicles (OMVs) as a direct pathway for delivering selected bacterial proteins and active compounds to the host. In fact, vesicles released by gut microbiota are emerging as key players in signaling processes in the intestinal mucosa. In the present study, the dextran sodium sulfate (DSS)-induced colitis mouse model was used to investigate the potential of EcN OMVs to ameliorate mucosal injury and inflammation in the gut. The experimental protocol involved pre-treatment with OMVs for 10 days before DSS intake, and a 5-day recovery period. Oral administration of purified EcN OMVs (5 μg/day) significantly reduced DSS-induced weight loss and ameliorated clinical symptoms and histological scores. OMVs treatment counteracted altered expression of cytokines and markers of intestinal barrier function. This study shows for the first time that EcN OMVs can mediate the anti-inflammatory and barrier protection effects previously reported for this probiotic in experimental colitis. Remarkably, translation of probiotics to human healthcare requires knowledge of the molecular mechanisms involved in probiotic-host interactions. Thus, OMVs, as a non-replicative bacterial form, could be explored as a new probiotic-derived therapeutic approach, with even lower risk of adverse events than probiotic administration.
PB Frontiers Research Foundation
SN 1664-302X
YR 2017
FD 2017-06-26
LK http://hdl.handle.net/10668/11441
UL http://hdl.handle.net/10668/11441
LA en
NO Fábrega MJ, Rodríguez-Nogales A, Garrido-Mesa J, Algieri F, Badía J, Giménez R, et al. Intestinal Anti-inflammatory Effects of Outer Membrane Vesicles from Escherichia coli Nissle 1917 in DSS-Experimental Colitis in Mice. Front Microbiol. 2017 Jul 11;8:1274.
DS RISalud
RD Apr 5, 2025