RT Journal Article
T1 Using Gene Editing Approaches to Fine-Tune the Immune System
A1 Pavlovic, Kristina
A1 Tristán-Manzano, María
A1 Maldonado-Pérez, Noelia
A1 Cortijo-Gutierrez, Marina
A1 Sánchez-Hernández, Sabina
A1 Justicia-Lirio, Pedro
A1 Carmona, M. Dolores
A1 Herrera, Concha
A1 Martin, Francisco
A1 Benabdellah, Karim
K1 Immunotherapy
K1 CARs
K1 Gene editing
K1 Graft-vs-host disease
K1 Base editors
K1 Inmunoterapia
K1 Receptores quiméricos de antígenos
K1 Edición génica
K1 Enfermedad injerto contra huésped
K1 Sistema inmunológico
AB Genome editing technologies not only provide unprecedented opportunities to study basic cellular system functionality but also improve the outcomes of several clinical applications. In this review, we analyze various gene editing techniques used to fine-tune immune systems from a basic research and clinical perspective. We discuss recent advances in the development of programmable nucleases, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR)-Cas-associated nucleases. We also discuss the use of programmable nucleases and their derivative reagents such as base editing tools to engineer immune cells via gene disruption, insertion, and rewriting of T cells and other immune components, such natural killers (NKs) and hematopoietic stem and progenitor cells (HSPCs). In addition, with regard to chimeric antigen receptors (CARs), we describe how different gene editing tools enable healthy donor cells to be used in CAR T therapy instead of autologous cells without risking graft-versus-host disease or rejection, leading to reduced adoptive cell therapy costs and instant treatment availability for patients. We pay particular attention to the delivery of therapeutic transgenes, such as CARs, to endogenous loci which prevents collateral damage and increases therapeutic effectiveness. Finally, we review creative innovations, including immune system repurposing, that facilitate safe and efficient genome surgery within the framework of clinical cancer immunotherapies.
PB Frontiers
YR 2020
FD 2020-09-29
LK http://hdl.handle.net/10668/3745
UL http://hdl.handle.net/10668/3745
LA en
NO Pavlovic K, Tristán-Manzano M, Maldonado-Pérez N, Cortijo-Gutierrez M, Sánchez-Hernández S, Justicia-Lirio P, et al. Using Gene Editing Approaches to Fine-Tune the Immune System. Front Immunol. 2020 Sep 29;11:570672
DS RISalud
RD Apr 5, 2025