RT Journal Article
T1 Breast cancer 1 (BrCa1) may be behind decreased lipogenesis in adipose tissue from obese subjects.
A1 Ortega, Francisco J
A1 Moreno-Navarrete, José M
A1 Mayas Torres, María Dolores
A1 García-Santos, Eva
A1 Gómez-Serrano, María
A1 Rodríguez-Hermosa, José I
A1 Ruiz, Bartomeu
A1 Ricart, Wifredo
A1 Tinahones, Francisco J
A1 Frühbeck, Gema
A1 Peral, Belén
A1 Fernández-Real, José M
K1 RNA, Messenger
K1 Acetyl-CoA Carboxylase
K1 BRCA1 Protein
K1 ARN Mensajero
K1 Acetil CoA Carboxilasa
K1 Proteína BRCA1
K1 Adipocitos
K1 Tejido Adiposo
K1 Diferenciación Celular
K1 Lipogénesis
K1 Macrófagos
K1 Obesidad
K1 Fosforilación oxidativa
K1 Células 3T3-L1
AB CONTEXTExpression and activity of the main lipogenic enzymes is paradoxically decreased in obesity, but the mechanisms behind these findings are poorly known. Breast Cancer 1 (BrCa1) interacts with acetyl-CoA carboxylase (ACC) reducing the rate of fatty acid biosynthesis. In this study, we aimed to evaluate BrCa1 in human adipose tissue according to obesity and insulin resistance, and in vitro cultured adipocytes.RESEARCH DESIGN AND METHODSBrCa1 gene expression, total and phosphorylated (P-) BrCa1, and ACC were analyzed in adipose tissue samples obtained from a total sample of 133 subjects. BrCa1 expression was also evaluated during in vitro differentiation of human adipocytes and 3T3-L1 cells.RESULTSBrCa1 gene expression was significantly up-regulated in both omental (OM; 1.36-fold, p = 0.002) and subcutaneous (SC; 1.49-fold, p = 0.001) adipose tissue from obese subjects. In parallel with increased BrCa1 mRNA, P-ACC was also up-regulated in SC (p = 0.007) as well as in OM (p = 0.010) fat from obese subjects. Consistent with its role limiting fatty acid biosynthesis, both BrCa1 mRNA (3.5-fold, p<0.0001) and protein (1.2-fold, p = 0.001) were increased in pre-adipocytes, and decreased during in vitro adipogenesis, while P-ACC decreased during differentiation of human adipocytes (p = 0.005) allowing lipid biosynthesis. Interestingly, BrCa1 gene expression in mature adipocytes was restored by inflammatory stimuli (macrophage conditioned medium), whereas lipogenic genes significantly decreased.CONCLUSIONSThe specular findings of BrCa1 and lipogenic enzymes in adipose tissue and adipocytes reported here suggest that BrCa1 might help to control fatty acid biosynthesis in adipocytes and adipose tissue from obese subjects.
PB Public Library of Science
YR 2012
FD 2012-05-30
LK http://hdl.handle.net/10668/663
UL http://hdl.handle.net/10668/663
LA en
NO Ortega FJ, Moreno-Navarrete JM, Mayas Torres MD, García-Santos E, Gómez-Serrano M, Rodriguez-Hermosa JI, et al. Breast cancer 1 (BrCa1) may be behind decreased lipogenesis in adipose tissue from obese subjects. PLoS ONE; 7(5):e33233
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Mar 14, 2025