RT Journal Article
T1 A Standardized Extract of Lentinula edodes Cultured Mycelium Inhibits Pseudomonas aeruginosa Infectivity Mechanisms.
A1 Tena-Garitaonaindia, Mireia
A1 Ceacero-Heras, Diego
A1 Montoro, Maria Del Mar Maldonado
A1 de Medina, Fermin Sanchez
A1 Martinez-Augustin, Olga
A1 Daddaoua, Abdelali
K1 AHCC®
K1 PCR real time (qPCR)
K1 Pseudomonas aeruginosa
K1 immune response
K1 internalization
K1 motility and biofilm
K1 prebiotic
K1 secretion system and adhesion
AB The priority pathogen list of the World Health Organization classified Pseudomonas aeruginosa as the second top critical pathogen. Hence, the development of novel antibacterial strategies to tackle this bacterium is highly necessary. Herein we explore the potential antibacterial effect of a standardized extract of cultured mycelium of Lentinula edodes (AHCC®) on P. aeruginosa. AHCC® was found to inhibit the growth rate and biofilm formation of strain PAO1. No change in swarming was observed, but AHCC® hampered swimming and twitching motility. In accordance, a decreased expression of metabolism, growth, and biofilm formation genes was shown. AHCC® also diminished the levels of exotoxin A and bacteria inside IEC18 cells and the secretion of IL-6, IL-10 and TNF by infected macrophages. This effect was related to a reduced phosphorylation of MAPKs and to bacteria internalization. Taken together, our data suggest that AHCC® has a potential role to prevent P. aeruginosa infections and may lead to the development of new therapies.
PB Frontiers Research Foundation
SN 1664-302X
YR 2022
FD 2022-02-14
LK http://hdl.handle.net/10668/20617
UL http://hdl.handle.net/10668/20617
LA en
NO Tena-Garitaonaindia M, Ceacero-Heras D, Montoro MDMM, de Medina FS, Martínez-Augustin O, Daddaoua A. A Standardized Extract of Lentinula edodes Cultured Mycelium Inhibits Pseudomonas aeruginosa Infectivity Mechanisms. Front Microbiol. 2022 Mar 16;13:814448
DS RISalud
RD Apr 6, 2025