RT Journal Article
T1 Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) Seeds.
A1 Jimenez-Lopez, Jose C
A1 Zafra, Adoración
A1 Palanco, Lucía
A1 Florido, José Fernando
A1 Alché, Juan de Dios
K1 Alérgenos
K1 Biología computacional
K1 ADN complementario
K1 Epítopos
K1 Nueces
K1 Olea
K1 Filogenia
K1 reacción en cadena de la polimerasa
K1 Estructura secundaria de proteína
K1 Estructura terciaria de proteína
K1 Proteínas de almacenamiento de semillas
K1 Semillas
K1 Transcriptoma
AB Olive seeds, which are a raw material of interest, have been reported to contain 11S seed storage proteins (SSPs). However, the presence of SSPs such as 7S vicilins has not been studied. In this study, following a search in the olive seed transcriptome, 58 sequences corresponding to 7S vicilins were retrieved. A partial sequence was amplified by PCR from olive seed cDNA and subjected to phylogenetic analysis with other sequences. Structural analysis showed that olive 7S vicilin contains 9 α-helixes and 22 β-sheets. Additionally, 3D structural analysis displayed good superimposition with vicilin models generated from Pistacia and Sesamum. In order to assess potential allergenicity, T and B epitopes present in these proteins were identified by bioinformatic approaches. Different motifs were observed among the species, as well as some species-specific motifs. Finally, expression analysis of vicilins was carried out in protein extracts obtained from seeds of different species, including the olive. Noticeable bands were observed for all species in the 15-75 kDa MW interval, which were compatible with vicilins. The reactivity of the extracts to sera from patients allergic to nuts was also analysed. The findings with regard to the potential use of olive seed as food are discussed.
PB Hindawi Publishing Corporation
YR 2016
FD 2016
LK http://hdl.handle.net/10668/2515
UL http://hdl.handle.net/10668/2515
LA en
NO Jimenez-Lopez JC, Zafra A, Palanco L, Florido JF, Alché Jde D. Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) Seeds. Biomed Res Int; 2016:4946872
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 14, 2025