RT Journal Article
T1 Epicatechin modulates stress-resistance in C. elegans via insulin/IGF-1 signaling pathway.
A1 Ayuda-Duran, Begoña
A1 Gonzalez-Manzano, Susana
A1 Miranda-Vizuete, Antonio
A1 Dueñas, Montserrat
A1 Santos-Buelga, Celestino
A1 Gonzalez-Paramas, Ana M
K1 Caenorhabditis elegans
K1 Lipid Peroxidation
K1 Reactive Oxygen Species
K1 Signal Transduction
AB The nematode Caenorhabditis elegans has been used to examine the influence of epicatechin (EC), an abundant flavonoid in the human diet, in some stress biomarkers (ROS production, lipid peroxidation and protein carbonylation). Furthermore, the ability of EC to modulate the expression of some key genes in the insulin/IGF-1 signaling pathway (IIS), involved in longevity and oxidative or heat shock stress response, has also been explored. The final aim was to contribute to the elucidation of the mechanisms involved in the biological effects of flavonoids. The results showed that EC-treated wild-type C. elegans exhibited increased survival and reduced oxidative damage of biomolecules when submitted to thermal stress. EC treatment led to a moderate elevation in ROS levels, which might activate endogenous mechanisms of defense protecting against oxidative insult. The enhanced stress resistance induced by EC was found to be mediated through the IIS pathway, since assays in daf-2, age-1, akt-1, akt-2, sgk-1, daf-16, skn-1 and hsf-1 loss of function mutant strains failed to show any heat-resistant phenotype against thermal stress when treated with EC. Consistently, EC treatment upregulated the expression of some stress resistance associated genes, such as gst-4, hsp-16.2 and hsp-70, which are downstream regulated by the IIS pathway.
PB Public Library of Science
YR 2019
FD 2019-01-28
LK http://hdl.handle.net/10668/13471
UL http://hdl.handle.net/10668/13471
LA en
NO Ayuda-Durán B, González-Manzano S, Miranda-Vizuete A, Dueñas M, Santos-Buelga C, González-Paramás AM. Epicatechin modulates stress-resistance in C. elegans via insulin/IGF-1 signaling pathway. PLoS One. 2019 Jan 28;14(1):e0199483.
NO This work was funded by MINECO (Spanish National Project AGL2015-64522-C2) (BFU2015-64408-P) and FEDER-Interreg España-Portugal Programme (Project ref. 0377_IBERPHENOL_6_E). B.A-D is recipient of PhD fellowships from the Junta de Castilla y Leon (Orden EDU/310/2015). The authors are thankful to Marta Rodríguez-Romero and José Antonio Mora-Lorca for providing the necessary help with the assays of RT-qPCR and fluorescence microscopy, respectively.
DS RISalud
RD Jun 1, 2025