%0 Journal Article
%A Zapatka, Mariel
%A Pociño-Merino, Irene
%A Heluani-Gahete, Hayat
%A Bermúdez-López, Marcelino
%A Tarrés, Marc
%A Ibars, Eva
%A Solé-Soler, Roger
%A Gutiérrez-Escribano, Pilar
%A Apostolova, Sonia
%A Casas, Celia
%A Aragon, Luis
%A Wellinger, Ralf
%A Colomina, Neus
%A Torres-Rosell, Jordi
%T Sumoylation of Smc5 Promotes Error-free Bypass at Damaged Replication Forks.
%D 2019
%U http://hdl.handle.net/10668/14788
%X Replication of a damaged DNA template can threaten the integrity of the genome, requiring the use of various mechanisms to tolerate DNA lesions. The Smc5/6 complex, together with the Nse2/Mms21 SUMO ligase, plays essential roles in genome stability through undefined tasks at damaged replication forks. Various subunits within the Smc5/6 complex are substrates of Nse2, but we currently do not know the role of these modifications. Here we show that sumoylation of Smc5 is targeted to its coiled-coil domain, is upregulated by replication fork damage, and participates in bypass of DNA lesions. smc5-KR mutant cells display defects in formation of sister chromatid junctions and higher translesion synthesis. Also, we provide evidence indicating that Smc5 sumoylation modulates Mph1-dependent fork regression, acting synergistically with other pathways to promote chromosome disjunction. We propose that sumoylation of Smc5 enhances physical remodeling of damaged forks, avoiding the use of a more mutagenic tolerance pathway.
%K DNA damage tolerance
%K DNA replication
%K Mms21
%K Mph1
%K Nse2
%K SUMO
%K Smc5
%K chromosome
%K fork regression
%K yeast
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