RT Journal Article
T1 Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia.
A1 Faura, Julia
A1 Ramiro, Laura
A1 Simats, Alba
A1 Ma, Feifei
A1 Penalba, Anna
A1 Gasull, Teresa
A1 Rosell, Anna
A1 Montaner, Joan
A1 Bustamante, Alejandro
K1 inflammation
K1 pulmonary complications
K1 stroke
AB After stroke and other brain injuries , there is a high incidence of respiratory complications such as pneumonia or acute lung injury . The molecular mechanisms that drive the brain - lung interaction post- stroke have not yet been elucidated. We performed transient middle cerebral artery occlusion (MCAO) and sham surgery on C57BL/6J mice and collected bronchoalveolar lavage fluid (BALF), serum , brain , and lung homogenate samples 24 h after surgery . A 92 proteins -panel developed by Olink Proteomics ® was used to analyze the content in BALF and lung homogenates. MCAO animals had higher protein concentration levels in BALF than sham-controls, but these levels did not correlate with the infarct volume. No alteration in alveolar- capillary barrier permeability was observed. A total of 12 and 14 proteins were differentially expressed between the groups (FDR < 0.1) in BALF and lung tissue homogenates, respectively. Of those, HGF, TGF-α, and CCL2 were identified as the most relevant to this study. Their protein expression patterns were verified by ELISA . This study confirmed that post- stroke lung damage was not associated with increased lung permeability or cerebral ischemia severity. Furthermore, the dysregulation of HGF, TGF-α, and CCL2 in BALF and lung tissue after ischemia could play an important role in the molecular mechanisms underlying stroke -induced lung damage.
PB MDPI AG
YR 2022
FD 2022-07-22
LK http://hdl.handle.net/10668/21178
UL http://hdl.handle.net/10668/21178
LA en
NO Faura J, Ramiro L, Simats A, Ma F, Penalba A, Gasull T, et al. Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia. Int J Mol Sci. 2022 Jul 22;23(15):8093.
DS RISalud
RD Oct 15, 2025