%0 Journal Article %A Faura, Julia %A Ramiro, Laura %A Simats, Alba %A Ma, Feifei %A Penalba, Anna %A Gasull, Teresa %A Rosell, Anna %A Montaner, Joan %A Bustamante, Alejandro %T Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia. %D 2022 %U http://hdl.handle.net/10668/21178 %X After stroke and other brain injuries , there is a high incidence of respiratory complications such as pneumonia or acute lung injury . The molecular mechanisms that drive the brain - lung interaction post- stroke have not yet been elucidated. We performed transient middle cerebral artery occlusion (MCAO) and sham surgery on C57BL/6J mice and collected bronchoalveolar lavage fluid (BALF), serum , brain , and lung homogenate samples 24 h after surgery . A 92 proteins -panel developed by Olink Proteomics ® was used to analyze the content in BALF and lung homogenates. MCAO animals had higher protein concentration levels in BALF than sham-controls, but these levels did not correlate with the infarct volume. No alteration in alveolar- capillary barrier permeability was observed. A total of 12 and 14 proteins were differentially expressed between the groups (FDR < 0.1) in BALF and lung tissue homogenates, respectively. Of those, HGF, TGF-α, and CCL2 were identified as the most relevant to this study. Their protein expression patterns were verified by ELISA . This study confirmed that post- stroke lung damage was not associated with increased lung permeability or cerebral ischemia severity. Furthermore, the dysregulation of HGF, TGF-α, and CCL2 in BALF and lung tissue after ischemia could play an important role in the molecular mechanisms underlying stroke -induced lung damage. %K inflammation %K pulmonary complications %K stroke %~