RT Journal Article
T1 Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons
A1 Carrascal, Livia
A1 Gorton, Ella
A1 Pardillo-Díaz, Ricardo
A1 Perez-García, Patricia
A1 Gómez-Oliva, Ricardo
A1 Castro, Carmen
A1 Nunez-Abades, Pedro
K1 Oxidative stress
K1 Motor cortex
K1 Glutathione levels in the brain
K1 Postnatal development
K1 Membrane excitability
K1 Amyotrophic lateral sclerosis
K1 Lipid peroxidation
K1 Estrés oxidativo
K1 Corteza motora
K1 Esclerosis amiotrófica lateral
K1 Peroxidación de lípido
AB Oxidative stress is one of the main proposed mechanisms involved in neuronal degeneration. To evaluate the consequences of oxidative stress on motor cortex pyramidal neurons during postnatal development, rats were classified into three groups: Newborn (P2-P7); infantile (P11-P15); and young adult (P20-P40). Oxidative stress was induced by 10 µM of cumene hydroperoxide (CH) application. In newborn rats, using the whole cell patch-clamp technique in brain slices, no significant modifications in membrane excitability were found. In infantile rats, the input resistance increased and rheobase decreased due to the blockage of GABAergic tonic conductance. Lipid peroxidation induced by CH resulted in a noticeable increase in protein-bound 4-hidroxynonenal in homogenates in only infantile and young adult rat slices. Interestingly, homogenates of newborn rat brain slices showed the highest capacity to respond to oxidative stress by dramatically increasing their glutathione and free thiol content. This increase correlated with a time-dependent increase in the glutathione reductase activity, suggesting a greater buffering capacity of newborn rats to resist oxidative stress. Furthermore, pre-treatment of the slices with glutathione monoethyl ester acted as a neuroprotector in pyramidal neurons of infantile rats. We conclude that during maturation, the vulnerability to oxidative stress in rat motor neurons increases with age.
PB MDPI
YR 2020
FD 2020-12-19
LK http://hdl.handle.net/10668/4072
UL http://hdl.handle.net/10668/4072
LA en
NO Carrascal L, Gorton E, Pardillo-Díaz R, Perez-García P, Gómez-Oliva R, Castro C, et al. Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons. Antioxidants. 2020 Dec 19;9(12):1307
DS RISalud
RD Apr 11, 2025