RT Journal Article
T1 Green Alga Ulva spp. Hydrolysates and Their Peptide Fractions Regulate Cytokine Production in Splenic Macrophages and Lymphocytes Involving the TLR4-NFκB/MAPK Pathways.
A1 Cian, Raul E
A1 Hernandez-Chirlaque, Cristina
A1 Gamez-Belmonte, Reyes
A1 Drago, Silvina R
A1 Sanchez de Medina, Fermin
A1 Martinez-Augustin, Olga
K1 NFκB
K1 TLR2
K1 TLR4
K1 bioactive peptides
K1 green seaweeds
K1 interleukin 10
AB Hydrolysates of food protein sources have immunomodulatory effects, which are of interest for use as functional foods. In this study, we have characterized the immune regulatory effect on rat splenocytes, macrophages and T lymphocytes of Ulva spp. hydrolysates and their peptide fractions with or without in vitro gastrointestinal digestion and/or ultrafiltration. IL-10 was induced in almost all conditions and cell types obtained from wild type animals. The induction was in general increased by ultrafiltration and in vitro gastrointestinal digestion. TNF was also induced in basal conditions. In turn, TNF and IFN-γ production was attenuated by the hydrolysate products in lipopolysaccharide or concanavalin A immune stimulated cells. Inhibitors for the activation of NFκB, MAPK p38 and JNK inhibited IL-10 induction in rat splenocytes. The response was dramatically attenuated in TLR4-/- cells, and only modestly in TLR2-/- cells. Food peptides from Ulva spp. genus exert anti-inflammatory effects in immune cells mediated by TLR4 and NFκB. Similarity with the immunomodulatory profile of protein hydrolysates from other sources suggests a common mechanism.
PB MDPI AG
YR 2018
FD 2018-07-10
LK http://hdl.handle.net/10668/12703
UL http://hdl.handle.net/10668/12703
LA en
NO Cian RE, Hernández-Chirlaque C, Gámez-Belmonte R, Drago SR, Sánchez de Medina F, Martínez-Augustin O. Green Alga Ulva spp. Hydrolysates and Their Peptide Fractions Regulate Cytokine Production in Splenic Macrophages and Lymphocytes Involving the TLR4-NFκB/MAPK Pathways. Mar Drugs. 2018 Jul 11;16(7):235.
DS RISalud
RD Apr 6, 2025