RT Journal Article
T1 Development and Preliminary Evaluation of a Rapid Oligochromatographic Assay for Specific Detection of New Human Influenza A H1N1 Virus
A1 Pérez-Ruiz, Mercedes
A1 Navarro-Marí, José María
A1 Bautista-Marín, María-Fé
A1 Pedrosa-Corral, Irene
A1 Sanbonmatsu-Gámez, Sara
A1 Camacho, Ana G.
A1 Rojas, José
A1 Ruiz-Ortiz, Jorge
A1 Rodríguez-Granger, Javier
A1 Carrillo, José Antonio
K1 Cromatografía
K1 Diagnóstico por imagen
K1 Hemaglutininas virales
K1 Humanos
K1 Virus de la Influenza A
K1 Gripe aviar
K1 Plásmidos
K1 ARN viral
K1 ADN Polimerasa dirigida por ARN
K1 Sensibilidad y especificidad
K1 Factores de tiempo
K1 Virología
K1 Reacción en cadena de la polimerasa de transcriptasa inversa
AB A new oligochromatographic assay, Speed-Oligo Novel Influenza A H1N1, was designed and optimized for the specific detection of the 2009 influenza A H1N1 virus. The assay is based on a PCR method coupled to detection of PCR products by means of a dipstick device. The target sequence is a 103-bp fragment within the hemagglutinin gene. The analytical sensitivity of the new assay was measured with serial dilutions of a plasmid that contained the target sequence, and we determined that down to one copy per reaction of the plasmid was reliably detected. Diagnostic performance was assessed with 103 RNAs from suspected cases (40 positive and 63 negative results) previously analyzed with a reference real-time PCR technique. All positive cases were confirmed, and no false-positive results were detected with the new assay. No cross-reactions were observed when other viral strains or clinical samples with other respiratory viruses were tested. According to these results, this new assay has 100% sensitivity and specificity. The turnaround time for the whole procedure was 140 min. The assay may be especially useful for the specific detection of 2009 H1N1 virus in laboratories not equipped with real-time PCR instruments
PB American Society for Microbiology
SN 0095-1137
YR 2010
FD 2010-05
LK http://hdl.handle.net/10668/2086
UL http://hdl.handle.net/10668/2086
LA en
NO Pérez-Ruiz M, Navarro-Marí JM, Bautista-Marín MF, Pedrosa-Corral I, Sanbonmatsu-Gámez S, Camacho AG et al. Development and Preliminary Evaluation of a Rapid Oligochromatographic Assay for Specific Detection of New Human Influenza A H1N1 Virus. J Clin Microbiol. 2010 May;48(5):1801-5
DS RISalud
RD Mar 14, 2025