RT Journal Article
T1 Investigating the Epigenetic Discrimination of Identical Twins Using Buccal Swabs, Saliva, and Cigarette Butts in the Forensic Setting.
A1 Vidaki, Athina
A1 Kalamara, Vivian
A1 Carnero-Montoro, Elena
A1 Spector, Timothy D
A1 Bell, Jordana T
A1 Kayser, Manfred
K1 DNA methylation
K1 Illumina 450K array
K1 MethyLight
K1 buccal cells
K1 cigarette butts
K1 epigenomics
K1 forensics
K1 individual identification
K1 monozygotic twins
K1 saliva
AB Monozygotic (MZ) twins are typically indistinguishable via forensic DNA profiling. Recently, we demonstrated that epigenetic differentiation of MZ twins is feasible; however, proportions of twin differentially methylated CpG sites (tDMSs) identified in reference-type blood DNA were not replicated in trace-type blood DNA. Here we investigated buccal swabs as typical forensic reference material, and saliva and cigarette butts as commonly encountered forensic trace materials. As an analog to a forensic case, we analyzed one MZ twin pair. Epigenome-wide microarray analysis in reference-type buccal DNA revealed 25 candidate tDMSs with >0.5 twin-to-twin differences. MethyLight quantitative PCR (qPCR) of 22 selected tDMSs in trace-type DNA revealed in saliva DNA that six tDMSs (27.3%) had >0.1 twin-to-twin differences, seven (31.8%) had smaller (0.5 twin-to-twin differences. MethyLight quantitative PCR (qPCR) of 22 selected tDMSs in trace-type DNA revealed in saliva DNA that six tDMSs (27.3%) had >0.1 twin-to-twin differences, seven (31.8%) had smaller (0.1 twin-to-twin differences, seven (31.8%) had smaller (
SN 2073-4425
YR 2018
FD 2018-05-14
LK http://hdl.handle.net/10668/12461
UL http://hdl.handle.net/10668/12461
LA en
DS RISalud
RD Apr 5, 2025