RT Journal Article
T1 Gut microbiota in children with type 1 diabetes differs from that in healthy children: a case-control study.
A1 Murri, Mora
A1 Leiva, Isabel
A1 Gómez-Zumaquero, Juan Miguel
A1 Tinahones, Francisco J
A1 Cardona, Fernando
A1 Soriguer, Federico
A1 Queipo-Ortuño, María Isabel
K1 butyrate-producing bacteria
K1 glycemic level
K1 gut integrity
K1 gut microbiota
K1 gut permeability
K1 HbA1c level
K1 lactic acid-producing bacteria
K1 mode of delivery
K1 mucin
K1 PCR-DGGE
K1 type 1 diabetes
K1 Permeabilidad
K1 Ácido Láctico
K1 Hemoglobina A Glucosilada
K1 Niño
K1 Intestino
K1 Diabetes Mellitus Tipo 1
K1 Ácido Butírico
AB BACKGROUNDA recent study using a rat model found significant differences at the time of diabetes onset in the bacterial communities responsible for type 1 diabetes modulation. We hypothesized that type 1 diabetes in humans could also be linked to a specific gut microbiota. Our aim was to quantify and evaluate the difference in the composition of gut microbiota between children with type 1 diabetes and healthy children and to determine the possible relationship of the gut microbiota of children with type 1 diabetes with the glycemic level.METHODSA case-control study was carried out with 16 children with type 1 diabetes and 16 healthy children. The fecal bacteria composition was investigated by polymerase chain reaction-denaturing gradient gel electrophoresis and real-time quantitative polymerase chain reaction.RESULTSThe mean similarity index was 47.39% for the healthy children and 37.56% for the children with diabetes, whereas the intergroup similarity index was 26.69%. In the children with diabetes, the bacterial number of Actinobacteria and Firmicutes, and the Firmicutes to Bacteroidetes ratio were all significantly decreased, with the quantity of Bacteroidetes significantly increased with respect to healthy children. At the genus level, we found a significant increase in the number of Clostridium, Bacteroides and Veillonella and a significant decrease in the number of Lactobacillus, Bifidobacterium, Blautia coccoides/Eubacterium rectale group and Prevotella in the children with diabetes. We also found that the number of Bifidobacterium and Lactobacillus, and the Firmicutes to Bacteroidetes ratio correlated negatively and significantly with the plasma glucose level while the quantity of Clostridium correlated positively and significantly with the plasma glucose level in the diabetes group.CONCLUSIONSThis is the first study showing that type 1 diabetes is associated with compositional changes in gut microbiota. The significant differences in the number of Bifidobacterium, Lactobacillus and Clostridium and in the Firmicutes to Bacteroidetes ratio observed between the two groups could be related to the glycemic level in the group with diabetes. Moreover, the quantity of bacteria essential to maintain gut integrity was significantly lower in the children with diabetes than the healthy children. These findings could be useful for developing strategies to control the development of type 1 diabetes by modifying the gut microbiota.
PB BioMed Central
YR 2013
FD 2013-02
LK http://hdl.handle.net/10668/916
UL http://hdl.handle.net/10668/916
LA en
NO Murri M, Leiva I, Gomez-Zumaquero JM, Tinahones FJ, Cardona F, Soriguer F, et al. Gut microbiota in children with type 1 diabetes differs from that in healthy children: a case-control study. BMC Med. 2013:11(1):46
NO Journal Article;
DS RISalud
RD Apr 9, 2025