RT Journal Article
T1 Human adipose tissue expresses intrinsic circadian rhythm in insulin sensitivity.
A1 Carrasco-Benso, Maria P
A1 Rivero-Gutierrez, Belen
A1 Lopez-Minguez, Jesus
A1 Anzola, Andrea
A1 Diez-Noguera, Antoni
A1 Madrid, Juan A
A1 Lujan, Juan A
A1 Martinez-Augustin, Olga
A1 Scheer, Frank A J L
A1 Garaulet, Marta
K1 diabetes
K1 glucose tolerance
K1 obesity
AB In humans, insulin sensitivity varies according to time of day, with decreased values in the evening and at night. Mechanisms responsible for the diurnal variation in insulin sensitivity are unclear. We investigated whether human adipose tissue (AT) expresses intrinsic circadian rhythms in insulin sensitivity that could contribute to this phenomenon. Subcutaneous and visceral AT biopsies were obtained from extremely obese participants (body mass index, 41.8 ± 6.3 kg/m(2); 46 ± 11 y) during gastric-bypass surgery. To assess the rhythm in insulin signaling, AKT phosphorylation was determined every 4 h over 24 h in vitro in response to different insulin concentrations (0, 1, 10, and 100 nM). Data revealed that subcutaneous AT exhibited robust circadian rhythms in insulin signaling (P
PB John Wiley & Sons, Inc.
YR 2016
FD 2016-05-19
LK http://hdl.handle.net/10668/10148
UL http://hdl.handle.net/10668/10148
LA en
NO Carrasco-Benso MP, Rivero-Gutierrez B, Lopez-Minguez J, Anzola A, Diez-Noguera A, Madrid JA, et al.  Human adipose tissue expresses intrinsic circadian rhythm in insulin sensitivity. FASEB J. 2016 Sep;30(9):3117-23.
NO This study was supported by Spanish Government of Economy and Competitiveness (Grant SAF2014–52480-R) and from European Regional Development Fund (ERDF) (to M.G.), Grant SAF2011–22812 (to O.M.-A.), the Seneca Foundation from the Government of Murcia (15123/PI/10; to M.G.), and by U.S. National Institutes of Health Grants R01-DK099512 (National Institute of Diabetes and Digestive and Kidney Diseases) and R01-HL118601 (National Heart, Lung, and Blood Institute) (to F.A.J.L.S.). Author contributions: M. P. Carrasco-Benso conducted the experiments, performed the cultures, analyzed data, and wrote the paper; B. Rivero-Gutierrez performed Western blot experiments; J. Lopez-Minguez performed the cultures; A. Anzola performed Western blot experiments; A. Diez-Noguera analyzed circadian data; J. A. Madrid gave advice for circadian aspects; J. A. Lujan recruited the study participants and performed the biopsies; O. Martínez-Augustin directed the Western blot experiments; and F. A. J. L. Scheer and M. Garaulet designed research, wrote the paper, and have primary responsibility for final content.
DS RISalud
RD Apr 7, 2025