RT Journal Article
T1 Transcriptional Profiling of Dendritic Cells in a Mouse Model of Food-Antigen-Induced Anaphylaxis Reveals the Upregulation of Multiple Immune-Related Pathways.
A1 Rodriguez, Maria Jose
A1 Palomares, Francisca
A1 Bogas, Gador
A1 Torres, Maria Jose
A1 Diaz-Perales, Araceli
A1 Rojo, Javier
A1 Plaza-Seron, Maria Del Carmen
A1 Rodriguez-Nogales, Alba
A1 Orengo, Christine
A1 Mayorga, Cristobalina
A1 Perkins, James Richard
K1 anaphylaxis
K1 dendritic cells
K1 food allergy
K1 lipid transfer proteins
K1 transcriptomics
AB Much of the knowledge about gene expression during anaphylaxis comes from candidate gene studies. Despite their potential role, expression changes in dendritic cells (DCs) have not been studied in this context using high throughput methods. The molecular mechanisms underlying food-antigen-induced anaphylaxis are investigated using DCs from an animal model. RNA sequencing is used to study gene expression in lymph-node-derived DCs from anaphylactic mice sensitized intranasally with the major peach allergen Pru p 3 during the acute reaction phase, induced intraperitoneally. In total, 237 genes changed significantly, 181 showing at least twofold changes. Almost three-quarters of these increase during anaphylaxis. A subset is confirmed using RT-PCR in a second set of samples obtained from a new batch of mice. Enrichment analysis shows an overrepresentation of genes involved in key immune system and inflammatory processes, including TGF-β signaling. Comparison with a study using anaphylactic human subjects show significant overlap. The findings provide a comprehensive overview of the transcriptional changes occurring in DCs during anaphylaxis and help elucidate the mechanisms involved. They add further weight to the putative role of these cells in anaphylaxis and highlight genes that may represent potential therapeutic targets.
YR 2018
FD 2018-12-17
LK http://hdl.handle.net/10668/13213
UL http://hdl.handle.net/10668/13213
LA en
DS RISalud
RD Apr 17, 2025