RT Journal Article
T1 A versatile workflow to integrate RNA-seq genomic and transcriptomic data into mechanistic models of signaling pathways
A1 Garrido-Rodriguez, Martín
A1 Lopez-Lopez, Daniel
A1 Ortuno, Francisco M.
A1 Peña-Chilet, María
A1 Muñoz, Eduardo
A1 Calzado, Marco A.
A1 Dopazo, Joaquin
K1 Transcriptome
K1 RNA massive sequencing
K1 RNA-seq
K1 Workflow
K1 Signaling pathways
K1 Transcriptoma
K1 Flujo de trabajo
K1 Secuenciación de escopeta completa del transcriptoma
AB MIGNON is a workflow for the analysis of RNA-Seq experiments, which not only efficiently manages the estimation of gene expression levels from raw sequencing reads, but also calls genomic variants present in the transcripts analyzed. Moreover, this is the first workflow that provides a framework for the integration of transcriptomic and genomic data based on a mechanistic model of signaling pathway activities that allows a detailed biological interpretation of the results, including a comprehensive functional profiling of cell activity. MIGNON covers the whole process, from reads to signaling circuit activity estimations, using state-of-the-art tools, it is easy to use and it is deployable in different computational environments, allowing an optimized use of the resources available.
PB Public Library of Science
YR 2021
FD 2021-02-11
LK http://hdl.handle.net/10668/4220
UL http://hdl.handle.net/10668/4220
LA en
NO Garrido-Rodriguez M, Lopez-Lopez D, Ortuno FM, Peña-Chilet M, Muñoz E, Calzado MA, et al. A versatile workflow to integrate RNA-seq genomic and transcriptomic data into mechanistic models of signaling pathways. PLoS Comput Biol. 2021 Feb 11;17(2):e1008748
DS RISalud
RD Apr 8, 2025