RT Journal Article
T1 Covalent Functionalization of PMMA Surface with L-3,4-Dihydroxyphenylalanine (L-DOPA) to Enhance its Biocompatibility and Adhesion to Corneal Tissue
A1 Sharifi, Roholah
A1 Mahmoudzadeh, Soudabe
A1 Islam, Mohammad Mirazul
A1 Koza, Darrell
A1 Dohlman, Claes H.
A1 Chodosh, James
A1 Gonzalez-Andrades, Miguel
K1 Biocompatibility
K1 Biointegration
K1 Boston-keratoprosthesis
K1 Cellular adhesion
K1 Cornea
K1 L-3
K1 4-dihydroxyphenylalanine
K1 Poly(methyl methacrylate)
AB The Boston keratoprosthesis (B-KPro) is globally the most commonly implanted artificial cornea for patients with severe corneal diseases, particularly those with multiple allograft failures. Despite providing a good visual recovery, the poor adhesion between the poly(methyl methacrylate) (PMMA)-made stem and the donor tissue poses a challenge, impacting the clinical outcome of the B-KPro. Using single-molecule covalent bonding, PMMA surface is functionalized with l-3,4-dihydroxyphenylalanine (l-DOPA) and its chemical, optical, mechanical, and biological properties are studied. The functionalization process significantly improves biocompatibility of PMMA, without affecting its optical and mechanical properties. Human corneal fibroblasts (HCF) and human corneal epithelial cells (HCEp) seeded on l-DOPA surface both exhibit greater confluency and metabolic rate compared to those of PMMA during 7-day cell culture. Moreover, HCF cultured on l-DOPA demonstrates a higher expression of ALDH3A1, Ki67, Integrin 1, and FAK with no expression of alpha-SMA, compared to those of PMMA, which instead show greater expression of alpha-SMA. These suggest that l-DOPA surface fosters cellular adhesion, proliferation, and migration, without adversely impacting the phenotype of the cells. This study offers an inexpensive and efficient tactic to modify the surface of materials with l-DOPA to achieve the optimal biocompatibility and biointegration of medical devices.
PB Wiley
SN 2196-7350
YR 2020
FD 2020-01-01
LK http://hdl.handle.net/10668/18603
UL http://hdl.handle.net/10668/18603
LA en
NO Sharifi R, Mahmoudzadeh S, Islam MM, Koza D, Dohlman CH, Chodosh J, et al. Covalent Functionalization of PMMA Surface with L‐3,4‐ Dihydroxyphenylalanine (L‐DOPA) to Enhance its Biocompatibility and Adhesion to Corneal Tissue. Advanced Materials Interfaces [Internet]. 5 de noviembre de 2019;7(1)
DS RISalud
RD Apr 12, 2025