TY - JOUR AU - Chato-Astrain, Jesus AU - Sanchez-Porras, David AU - Garcia-Garcia, Oscar Dario AU - Vairo, Claudia AU - Villar-Vidal, Maria AU - Villullas, Silvia AU - Sanchez-Montesinos, Indalecio AU - Campos, Fernando AU - Garzon, Ingrid AU - Alaminos, Miguel PY - 2021 DO - 10.3390/biomedicines9111634 UR - https://hdl.handle.net/10668/28038 T2 - Biomedicines AB - Human skin keratinocyte primary cultures can be established from skin biopsies with culture media containing epithelial growth factor (EGF). Although current methods are efficient, optimization is required to accelerate the procedure and obtain these... LA - en PB - Mdpi KW - EGF KW - nanoparticles KW - NLC KW - keratinocytes KW - tissue engineering KW - cell culture KW - Epidermal-growth-factor KW - In-vitro KW - Factor receptor KW - Nanoparticles KW - Rhegf KW - Differentiation KW - Proliferation KW - Dressings KW - Delivery KW - Wounds TI - Improvement of Cell Culture Methods for the Successful Generation of Human Keratinocyte Primary Cell Cultures Using EGF-Loaded Nanostructured Lipid Carriers TY - research article VL - 9 ER -