RT Journal Article
T1 Analytical performance of the Alere™ i Influenza A&B assay for the rapid detection of influenza viruses.
A1 Riazzo, Cristina
A1 Pérez-Ruiz, Mercedes
A1 Sanbonmatsu-Gámez, Sara
A1 Pedrosa-Corral, Irene
A1 Gutiérrez-Fernández, José
A1 Navarro-Marí, José-María
K1 Detección rápida
K1 Gripe
K1 Influenza
K1 Molecular testing
K1 Método molecular
K1 Rapid detection
AB The analytical performance of the new Alere™ i Influenza A&B kit (AL-Flu) assay, based on isothermal nucleic acids amplification, was evaluated and compared with an antigen detection method, SD Bioline Influenza Virus Antigen Test (SDB), and an automated real-time RT-PCR, Simplexa™ Flu A/B & VRS Direct assay (SPX), for detection of influenza viruses. An "in-house" RT-PCR was used as the reference method. Sensitivity of AL-Flu, SDB, and SPX was 71.7%, 34.8%, and 100%, respectively. Specificity was 100% for all techniques. The turnaround time was 13min for AL-Flu, 15min for SDB, and 75min for SPX. The Alere™ i Influenza A&B assay is an optimal point-of-care assay for influenza diagnosis in clinical emergency settings, and is more sensitive and specific than antigen detection methods.
YR 2015
FD 2015-11-25
LK http://hdl.handle.net/10668/9647
UL http://hdl.handle.net/10668/9647
LA en
LA es
DS RISalud
RD Apr 18, 2025