RT Journal Article
T1 Pru p 3-Glycodendropeptides Based on Mannoses Promote Changes in the Immunological Properties of Dendritic and T-Cells from LTP-Allergic Patients.
A1 Palomares, Francisca
A1 Ramos-Soriano, Javier
A1 Gomez, Francisca
A1 Mascaraque, Ainhoa
A1 Bogas, Gador
A1 Perkins, James Richard
A1 Gonzalez, Miguel
A1 Torres, Maria Jose
A1 Diaz-Perales, Araceli
A1 Rojo, Javier
A1 Mayorga, Cristobalina
K1 C-type lectin receptors
K1 dendritic cells
K1 food allergies
K1 lymphocytes
K1 mannose
AB Glycodendropeptides (GDPs) functionalized with mannose can enhance allergen interaction with dendritic cells (DCs) via C-type lectin receptors (CLRs), modulating the immune response. They can present multiple peptides and have potential applications for diagnosis and treatment of food allergy (FA). The immune response induced by GDPs with mannose and Pru p 3 peptides (mono/tetravalent) with ester (D1 ManPrup3/D4 ManPrup3) or ether linkers (D1 Man-O- Prup3/D4 Man-O- Prup3) in lipid-transfer-protein-allergic patients and tolerant controls is analyzed. The immunological response induced by GDPs is studied by assessing monocyte-derived-DC maturation, lymphocyte proliferation, cytokine production, and basophil response by flow cytometry. Dn ManPrup3 was recognized by DCs via CLRs inducing DC maturation in all subjects. However, CCR7 expression is significantly upregulated in allergic patients compared to tolerant controls. These changes correlate with lymphocyte proliferation and specific production of Th2/Th1 cytokines in allergic patients. Moreover, D1 ManPrup3 does not induce basophil activation. Dn ManPrup3 induces changes in DC maturation and lymphocyte proliferation, indicating specific recognition via CLRs. Prup3-GDPs are recognized by immune cells, inducing a specific immune response and modulating the immunological response in FA patients. The specific geometry of D1 ManPrup3 in particular makes it a potential candidate for specific immunotherapy development.
YR 2019
FD 2019-08-08
LK http://hdl.handle.net/10668/14350
UL http://hdl.handle.net/10668/14350
LA en
DS RISalud
RD Apr 19, 2025