RT Journal Article
T1 Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes
A1 Hernández-Chirlaque, Cristina
A1 Aranda, Carlos J.
A1 Ocón, Borja
A1 Polo, Javier
A1 Martínez-Augustin, Olga
A1 Sánchez de Medina, Fermín
K1 Immunoglobulin concentrate
K1 Serum protein concentrate
K1 Toll-like receptors
K1 TLR4
K1 Myd88
K1 Inmunoglobulinas
K1 Proteínas sanguíneas
K1 Receptores similares a toll
K1 Receptor 4 similar a toll
K1 Factor 88 de diferenciación mieloide
AB Serum protein concentrates have been shown to exert in vivo anti-inflammatory effects. Specific effects on different cell types and their mechanism of action remain unraveled. We aimed to characterize the immunomodulatory effect of two porcine plasma protein concentrates, spray dried serum (SDS) and an immunoglobulin concentrate (IC), currently used as animal nutritional supplements with established in vivo immunomodulatory properties. Cytokine production by the intestinal epithelial cell line IEC18 and by primary cultures of rat splenocytes was studied. The molecular pathways involved were explored with specific inhibitors and gene knockdown. Our results indicate that both products induced GROα and MCP-1 production in IEC18 cells by a MyD88/NF-κB-dependent mechanism. Inhibition of TNF production was observed in rat primary splenocyte cultures. The immunoglobulin concentrate induced IL-10 expression in primary splenocytes and lymphocytes. The effect on TNF was independent of IL-10 production or the stimulation of NF-kB, MAPKs, AKT, or RAGE. In conclusion, SDS and IC directly regulate intestinal and systemic immune response in murine intestinal epithelial cells and in T lymphocytes and monocytes.
PB MDPI
YR 2021
FD 2021-03-13
LK http://hdl.handle.net/10668/4056
UL http://hdl.handle.net/10668/4056
LA en
NO Hernández-Chirlaque C, Aranda CJ, Ocón B, Polo J, Martínez-Augustin O, Sánchez de Medina F. Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes. Animals. 2021 Mar 13;11(3):807
DS RISalud
RD Apr 15, 2025