RT Journal Article
T1 Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency.
A1 Lebrón, José Antonio
A1 López-Cornejo, Pilar
A1 García-Dionisio, Elena
A1 Huertas, Pablo
A1 García-Calderón, Margarita
A1 Moyá, María Luisa
A1 Ostos, Francisco José
A1 López-López, Manuel
K1 DNA compacting efficiency
K1 cationic surfactants
K1 gene transfection
K1 lipoplexes
K1 liposomes
AB The interaction between calf-thymus DNA, ctDNA, and various single-chained surfactants with different functional groups at the end of hydrophobic tail was studied with the goal of investigating the influence of the functional group nature on surfactant DNA compacting efficiency. The surfactants investigated were dodecyltriethylammonium bromide (DTEABr), triethyl(1-phenoxydodecyl)ammonium bromide (12PhBr), triethyl(2-naphthoxydodecyl)ammonium bromide (12NBr) and 11-(isonicotinoyloxy)-N,N,N-triethyl-1-undecanaminium bromide (11PyBr). Results made evident that the surfactants' tendencies to self-aggregation is the key factor determining their efficiency to compact the nucleic acid. Subsequently, DOPE/12NBr/pEGFP-C1 lipoplexes, with different cationic surfactant molar fractions (α) and mass ratios (L/D), were prepared and characterized. DOPE is a zwitterionic phospholipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, and the plasmid pEGFP-C1 carries a GFP coding sequence with the necessary regulatory elements for constitutive expression of the gene in human cells. 12NBr was chosen because it was the most efficient DNA compacting agent among the surfactants investigated. Finally, the cytotoxicity and transfection efficiency (TE) of DOPE/12NBr/pDNA lipoplexes, with different compositions, were investigated.
SN 1999-4923
YR 2021
FD 2021-04-20
LK http://hdl.handle.net/10668/17714
UL http://hdl.handle.net/10668/17714
LA en
DS RISalud
RD Apr 7, 2025