RT Journal Article
T1 Novel bead-based platform for direct detection of unlabelled nucleic acids through Single Nucleobase Labelling.
A1 Venkateswaran, Seshasailam
A1 Luque-González, Maria Angélica
A1 Tabraue-Chávez, Mavys
A1 Fara, Mario Antonio
A1 López-Longarela, Barbara
A1 Cano-Cortes, Victoria
A1 López-Delgado, Francisco Javier
A1 Sánchez-Martín, Rosario María
A1 Ilyine, Hugh
A1 Bradley, Mark
A1 Pernagallo, Salvatore
A1 Díaz-Mochón, Juan José
K1 Dynamic chemistry
K1 Magnetic microspheres
K1 MicroRNA (miRNA)
K1 Nucleic acid
K1 Nucleic acid test (NAT)
K1 Peptide nucleic acid (PNA)
K1 Single Nucleobase Labelling (SNL)
AB Over the last decade, circulating microRNAs have received attention as diagnostic and prognostic biomarkers. In particular, microRNA122 has been demonstrated to be an early and more sensitive indicator of drug-induced liver injury than the widely used biomarkers such as alanine aminotransferase and aspartate aminotransferase. Recently, microRNA122 has been used in vitro to assess the cellular toxicity of new drugs and as a biomarker for the development of a rapid test for drug overdose/liver damage. In this proof-of-concept study, we report a PCR-free and label-free detection method that has a limit of detection (3 standard deviations) of 15 fmoles of microRNA122, by integrating a dynamic chemical approach for "Single Nucleobase Labelling" with a bead-based platform (Luminex®) thereby, in principle, demonstrating the exciting prospect of rapid and accurate profiling of any microRNAs related to diseases and toxicology.
YR 2016
FD 2016-08-31
LK http://hdl.handle.net/10668/10554
UL http://hdl.handle.net/10668/10554
LA en
DS RISalud
RD Apr 9, 2025