RT Journal Article
T1 Collagen analogs with phosphorylcholine are inflammation-suppressing scaffolds for corneal regeneration from alkali burns in mini-pigs.
A1 Simpson, Fiona C
A1 McTiernan, Christopher D
A1 Islam, Mohammad Mirazul
A1 Buznyk, Oleksiy
A1 Lewis, Philip N
A1 Meek, Keith M
A1 Haagdorens, Michel
A1 Audiger, Cindy
A1 Lesage, Sylvie
A1 Gueriot, François-Xavier
A1 Brunette, Isabelle
A1 Robert, Marie-Claude
A1 Olsen, David
A1 Koivusalo, Laura
A1 Liszka, Aneta
A1 Fagerholm, Per
A1 Gonzalez-Andrades, Miguel
A1 Griffith, May
K1 Alkalies
K1 Animals
K1 Biocompatible materials
K1 Burns, chemical
AB The long-term survival of biomaterial implants is often hampered by surgery-induced inflammation that can lead to graft failure. Considering that most corneas receiving grafts are either pathological or inflamed before implantation, the risk of rejection is heightened. Here, we show that bioengineered, fully synthetic, and robust corneal implants can be manufactured from a collagen analog (collagen-like peptide-polyethylene glycol hybrid, CLP-PEG) and inflammation-suppressing polymeric 2-methacryloyloxyethyl phosphorylcholine (MPC) when stabilized with the triazine-based crosslinker 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride. The resulting CLP-PEG-MPC implants led to reduced corneal swelling, haze, and neovascularization in comparison to CLP-PEG only implants when grafted into a mini-pig cornea alkali burn model of inflammation over 12 months. Implants incorporating MPC allowed for faster nerve regeneration and recovery of corneal sensation. CLP-PEG-MPC implants appear to be at a more advanced stage of regeneration than the CLP-PEG only implants, as evidenced by the presence of higher amounts of cornea-specific type V collagen, and a corresponding decrease in the presence of extracellular vesicles and exosomes in the corneal stroma, in keeping with the amounts present in healthy, unoperated corneas.
PB Nature Publishing Group
YR 2021
FD 2021-03-16
LK http://hdl.handle.net/10668/17819
UL http://hdl.handle.net/10668/17819
LA en
NO Simpson FC, McTiernan CD, Islam MM, Buznyk O, Lewis PN, Meek KM, et al. Collagen analogs with phosphorylcholine are inflammation-suppressing scaffolds for corneal regeneration from alkali burns in mini-pigs. Commun Biol. 2021 May 21;4(1):608
NO The GLP mini-pig study (AB16-07) was funded by a DBT-Vinnova Indo-Swedish strategic cooperative grant DNR 2013-04645, and conducted by Adlego AB. Funding for dendritic cell studies, data analyses was from CIHR grant 391487 to M.G. and I.B., Fonds de recherche en ophtalmologie de l’Université de Montréal (FROUM) to M.G. and SL, and a Caroline Durand Foundation Research Chair for Cellular Therapy in the Eye to M.G. F.C.S. was supported by a doctoral studentship from the Natural Sciences and Engineering Research Council of Canada (NSERC). K.M.M. and P.L. are funded by Programme Grant MR/S037829/1 from the U.K. Medical Research Council. M.G-A. acknowledges funding from ISCIII (ICI19/00006), co-funded by ERDF/ESF, “A way to make Europe”/“Investing in your future”). M.G. holds the Tier 1 Canada Research Chair for Biomaterials and Stem Cells in Ophthalmology.
DS RISalud
RD Apr 7, 2025