RT Journal Article
T1 Serum Cytokine Profiles of Melanoma Patients and Their Association with Tumor Progression and Metastasis
A1 Wang, Xu
A1 Montoyo-Pujol, Yoel Genaro
A1 Bermudez, Sandra
A1 Corpas, Gonzalo
A1 Martin, Aurelio
A1 Almazan, Francisco
A1 Cabrera, Teresa
A1 López-Nevot, Miguel Angel
K1 Vascular endothelial growth factor A
K1 Factor A de Crecimiento Endotelial Vascular
K1 Brain-derived neurotrophic factor
K1 Factor neurotrófico derivado del encéfalo
K1 BDNF
K1 Epidermal growth factor
K1 Factor de crecimiento epidérmico
K1 EGF
K1 Cytokines
K1 Citocinas
K1 Chemokines
K1 Quimiocinas
K1 Melanoma
AB Purpose: Previous studies have shown that melanoma cells produce excessive levels of cytokines, which have various biological roles during melanoma development. The aim of this study was to expand the profile of serum cytokines, chemokines, growth factors, and angiogenic factors that are associated with melanoma, to find more cytokines with abnormal concentrations in melanoma patients, to identify whether the level of cytokines correlated with prognostic variants, such as Breslow thickness and BRAF mutation, and, finally, to find out the cytokines that play important roles during melanoma development. Materials and methods: Multiplex immunobead assay technology and 45-plex immunoassays ProcartaPlex™ kits were used to simultaneously compare the levels of cytokines, growth factors, angiogenic factors, and chemokines between the serum of healthy patients (n = 30) and those with melanoma (n = 72). Data were analyzed according to the clinical characteristics of the designated patient subgroups.Results: Compared to the control group, melanoma patients had higher levels of VEGF-A, PDGF-BB, IL-1RA, PIGF-1, IFN-γ, TNF-α, MIP-1α, and SCF, but lower levels of BDNF, SDF-1α, MCP-1, Eotaxin, EGF, and IL-7. Furthermore, the levels of TNF-α (P=0.320, r = 0.019), IFN-γ (P=0.311, r = 0.023), VEGF-A (P=0.014, r = 0.337), and BDNF (0.004, r = -0.391) showed a significant correlation with Breslow thickness. IL-7 was of lower levels in patients harboring BRAF mutants. Melanoma patients with high levels of MIP-1α and MCP-1 showed the poorest overall survival. Conclusions: We found that the levels of VEGF-A and PDGF-BB in the serum of both primary and metastatic melanoma patients are elevated. TNF-α, IFN-γ, and VEGF-A presented a positive correlation with Breslow thickness, whereas BDNF showed a negative association. MIP-1α and MCP-1 correlated negatively with survival. In addition, lower levels of IL-7 were found in patients harboring BRAF mutants. These findings indicate that these cytokines may play critical roles in the progression of melanoma.
PB Hindawi Limited
SN 1687-8450
YR 2021
FD 2021-01-28
LK http://hdl.handle.net/10668/3987
UL http://hdl.handle.net/10668/3987
LA en
NO Wang X, Montoyo-Pujol YG, Bermudez S, Corpas G, Martin A, Almazan F. Serum Cytokine Profiles of Melanoma Patients and Their Association with Tumor Progression and Metastasis. J Oncol. 2021 Jan 28;2021:6610769
DS RISalud
RD Apr 8, 2025