RT Journal Article
T1 Bioactive Properties of the Aqueous Extracts of Endophytic Fungi Associated with Scots Pine (Pinus sylvestris) Roots.
A1 Tienaho, Jenni
A1 Karonen, Maarit
A1 Muilu-Mäkelä, Riina
A1 Kaseva, Janne
A1 de Pedro, Nuria
A1 Vicente, Francisca
A1 Genilloud, Olga
A1 Aapola, Ulla
A1 Uusitalo, Hannu
A1 Vuolteenaho, Katriina
A1 Franzén, Robert
A1 Wähälä, Kristiina
A1 Karp, Matti
A1 Santala, Ville
A1 Sarjala, Tytti
AB Despite the continuing interest in various plant and natural products, only a small portion of the biologically active compounds from nature has been discovered and exploited. In this study, antioxidant and antibacterial properties of aqueous fractions of three endophytic fungi isolated from the roots of 8-year-old Scots pines (Pinus sylvestris) growing on a drained peatland were investigated. The endophytic fungi species were Acephala applanata, Phialocephala fortinii, and Humicolopsis cephalosporioides/Coniochaeta mutabilis. The bioactivities were examined using hydrogen peroxide scavenging and oxygen radical absorbance capacity tests as well as sensitive Escherichia coli-based biosensors, which produce a luminescent signal in the presence of substances with oxidative or genotoxic properties. In addition, cell models for Parkinson's disease, age-related macular degeneration, and osteoarthritis were used to evaluate the potential for pharmaceutical applications. The aqueous extracts of fungi and 19 out of 42 fractions were found to be active in one or more of the tests used. However, no activity was found in the age-related macular degeneration and osteoarthritis cell model tests. Additionally, bioactivity data was connected with metabolites putatively annotated, and out of 330 metabolites, 177 were interesting in view of the bioactivities investigated. A majority of these were peptides and all three fungal species shared a highly similar metabolome. We propose that Scots pine endophytic fungi are a rich source of interesting metabolites, and synergistic effects may cause the bioactivities, as they were found to vary after the fractionation process.
YR 2020
FD 2020-06-10
LK http://hdl.handle.net/10668/15717
UL http://hdl.handle.net/10668/15717
LA en
DS RISalud
RD Apr 17, 2025