RT Journal Article
T1 Lymph Node Tumor Burden Correlates With Tumor Budding and Poorly Differentiated Clusters: A New Prognostic Factor in Colorectal Carcinoma?
A1 Archilla, Ivan
A1 Díaz-Mercedes, Sherley
A1 Aguirre, José Javier
A1 Tarragona, Jordi
A1 Machado, Isidro
A1 Rodrigo, Maria Teresa
A1 Lopez-Prades, Sandra
A1 Gorostiaga, Iñigo
A1 Landolfi, Stefania
A1 Alén, Begoña Otero
A1 Balaguer, Francesc
A1 Castells, Antoni
A1 Camps, Jordi
A1 Cuatrecasas, Miriam
AB Molecular lymph node (LN) staging in early colorectal cancer (CRC) has demonstrated to be more precise than conventional histopathology pN staging. Tumor budding (TB) and poorly differentiated clusters (PDCs) are associated with LN metastases, recurrences, and lower survival in CRC. We evaluated the correlation between the total tumor load (TTL) in LNs from CRC surgical specimens with patient outcome, TB, and PDC. In this retrospective multicentre study, 5,931 LNs from 342 stage I-III CRC were analyzed by both hematoxylin and eosin and molecular detection of tumor cytokeratin 19 mRNA by one-step nucleic acid amplification. TB and PDC were evaluated by hematoxylin and eosin and cytokeratin 19 immunohistochemistry. One-step nucleic acid was positive in 38.3% patients (n = 131). Tumor Budding was low in 45% cases, intermediate in 25%, and high in 30%. Poorly Differentiated Clusters were low-grade G1 in 53%, G2 in 32%, and G3 in 15%. TB and PDC correlated with TTL, high-grade, lymphovascular and perineural invasion, pT, pN and stage (P  The implementation of more sensitive molecular methods to assess LN status is a promising alternative approach to pN staging, which could be integrated to other factors to help risk stratification and management of patients with early-stage CRC. This study demonstrates the correlation of the amount of LN tumor burden with TB and PDCs. TTL is related to the outcome and could be used as a new prognostic factor in CRC (see Visual Abstract, Supplementary Digital Content 2, http://links.lww.com/CTG/A512).
YR 2021
FD 2021
LK http://hdl.handle.net/10668/17738
UL http://hdl.handle.net/10668/17738
LA en
DS RISalud
RD Apr 5, 2025