RT Journal Article
T1 Deregulation of miR-324/KISS1/kisspeptin in early ectopic pregnancy: mechanistic findings with clinical and diagnostic implications.
A1 Romero-Ruiz, Antonio
A1 Avendaño, Maria S
A1 Dominguez, Francisco
A1 Lozoya, Teresa
A1 Molina-Abril, Helena
A1 Sangiao-Alvarellos, Susana
A1 Gurrea, Marta
A1 Lara-Chica, Maribel
A1 Fernandez-Sanchez, Manuel
A1 Torres-Jimenez, Encarnación
A1 Perdices-Lopez, Cecilia
A1 Abbara, Ali
A1 Steffani, Liliana
A1 Calzado, Marco A
A1 Dhillo, Waljit S
A1 Pellicer, Antonio
A1 Tena-Sempere, Manuel
K1 KISS1
K1 Biomarker
K1 Diagnosis
K1 Ectopic pregnancy
K1 Kisspeptins
K1 miR-324-3p
AB Ectopic pregnancy is a life-threatening condition for which novel screening tools that would enable early accurate diagnosis would improve clinical outcomes. Kisspeptins, encoded by KISS1, play an essential role in human reproduction, at least partially by regulating placental function and possibly embryo implantation. Kisspeptin levels are elevated massively in normal pregnancy and reportedly altered in various gestational pathologic diseases. Yet, the pathophysiologic role of KISS1/kisspeptin in ectopic pregnancy has not been investigated previously. The purpose of this study was to evaluate changes of KISS1/kisspeptin levels in ectopic pregnancy and their underlaying molecular mechanisms and to ascertain the diagnostic implications of these changes. A total of 122 women with normal pregnancy who underwent voluntary termination of pregnancy and 84 patients who experienced tubal ectopic pregnancy were recruited. Measurements of plasma kisspeptins and KISS1 expression analyses in human embryonic/placental tissue were conducted in ectopic pregnancy and voluntary termination of pregnancy control subjects during the early gestational window ( Circulating kisspeptins gradually increased during the first trimester of normal pregnancy but were reduced markedly in ectopic pregnancy. This profile correlated with the expression levels of KISS1 in human embryonic/placental tissue, which increased in voluntary termination of pregnancy but remained suppressed in ectopic pregnancy. Bioinformatic predictions and expression analyses identified miR-27b-3p and miR-324-3p as putative repressors of KISS1 in human embryonic/placental tissue at  Our results document a significant down-regulation of KISS1/kisspeptins in early stages of ectopic pregnancy via, at least partially, a repressive interaction with miR-324-3p. Our data identify circulating kisspeptins and miR-324-3p as putative biomarkers for accurate screening of ectopic pregnancy at early gestational ages.
PB Elsevier
YR 2019
FD 2019-01-23
LK http://hdl.handle.net/10668/13494
UL http://hdl.handle.net/10668/13494
LA en
NO Romero-Ruiz A, Avendaño MS, Dominguez F, Lozoya T, Molina-Abril H, Sangiao-Alvarellos S, et al. Deregulation of miR-324/KISS1/kisspeptin in early ectopic pregnancy: mechanistic findings with clinical and diagnostic implications. Am J Obstet Gynecol. 2019 May;220(5):480.e1-480.e17
DS RISalud
RD Apr 7, 2025