RT Journal Article
T1 Maternal urinary concentrations of bisphenol A during pregnancy are associated with global DNA methylation in cord blood of newborns in the "NELA" birth cohort.
A1 Navarro-Lafuente, Fuensanta
A1 Adoamnei, Evdochia
A1 Arense-Gonzalo, Julian J
A1 Prieto-Sanchez, María T
A1 Sanchez-Ferrer, Maria L
A1 Parrado, Antonio
A1 Fernandez, Mariana F
A1 Suarez, Beatriz
A1 Lopez-Acosta, Antonia
A1 Sanchez-Guillamon, Antonio
A1 Garcia-Marcos, Luis
A1 Morales, Eva
A1 Mendiola, Jaime
A1 Torres-Cantero, Alberto M
A1 NELA Study group, 
K1 Bisphenol A
K1 DNA methylation
K1 Endocrine disruptors
K1 LINE-1
K1 Perinatal exposures
AB Endocrine disrupting chemicals (EDCs) set a public health risk through disruption of normal physiological processes. The toxicoepigenetic mechanisms of developmental exposure to common EDCs, such as bisphenol A (BPA), are poorly known. The present study aimed to evaluate associations between perinatal maternal urinary concentrations of BPA, bisphenol S (BPS) and bisphenol F (BPF) and LINE-1 (long interspersed nuclear elements) and Alu (short interspersed nuclear elements, SINEs) DNA methylation levels in newborns, as surrogate markers of global DNA methylation. Data come from 318 mother-child pairs of the `Nutrition in Early Life and Asthma´ (NELA) birth cohort. Urinary bisphenol concentration was measured by dispersive liquid-liquid microextraction and ultrahigh performance liquid chromatography with tandem mass spectrometry detection. DNA methylation was quantitatively assessed by bisulphite pyrosequencing on 3 LINEs and 5 SINEs. Unadjusted linear regression analyses showed that higher concentration of maternal urinary BPA in 24th week's pregnancy was associated with an increase in LINE-1 methylation in all newborns (p = 0.01) and, particularly, in male newborns (p = 0.03). These associations remained in full adjusted models [beta = 0.09 (95 % CI = 0.03; 0.14) for all newborns; and beta = 0.10 (95 % CI = 0.03; 0.17) for males], including a non-linear association for female newborns as well (p-trend = 0.003). No associations were found between maternal concentrations of bisphenol and Alu sequences. Our results suggest that exposure to environmental levels of BPA may be associated with a modest increase in LINE-1 methylation -as a relevant marker of epigenomic stability- during human fetal development. However, any effects on global DNA methylation are likely to be small, and of uncertain biological significance.
PB Elsevier BV
YR 2022
FD 2022-06-03
LK http://hdl.handle.net/10668/22512
UL http://hdl.handle.net/10668/22512
LA en
NO Navarro-Lafuente F, Adoamnei E, Arense-Gonzalo JJ, Prieto-Sánchez MT, Sánchez-Ferrer ML, Parrado A, et al. Maternal urinary concentrations of bisphenol A during pregnancy are associated with global DNA methylation in cord blood of newborns in the "NELA" birth cohort. Sci Total Environ. 2022 Sep 10;838(Pt 4):156540.
NO This work was supported by Instituto de Salud Carlos III (ISCIII), Spanish Ministry of Science, Innovation and Universities, Fondos FEDER (MS14/00046; CP14/00046; PIE15/00051; PI16/00422; FI17/00086; PI19/00863); and Fundación Séneca, Agencia de Ciencia y Tecnología Región de Murcia (20877/PI/18). Navarro-Lafuente F. is a recipient of a FPU Predoctoral fellowship from the Spanish Ministry of Science and Innovation (FPU18/01990). Morales E. was funded by Miguel Servet grants (MS14/00046 and CPII19/00019) awarded by the ISCIII, Spanish Ministry of Science, Innovation and Universities, and Fondos FEDER. Funders have no influence of any kind on analyses or results interpretations.
DS RISalud
RD Apr 15, 2025