%0 Journal Article
%A Navarro-Lafuente, Fuensanta
%A Adoamnei, Evdochia
%A Arense-Gonzalo, Julian J
%A Prieto-Sanchez, María T
%A Sanchez-Ferrer, Maria L
%A Parrado, Antonio
%A Fernandez, Mariana F
%A Suarez, Beatriz
%A Lopez-Acosta, Antonia
%A Sanchez-Guillamon, Antonio
%A Garcia-Marcos, Luis
%A Morales, Eva
%A Mendiola, Jaime
%A Torres-Cantero, Alberto M
%A NELA Study group
%T Maternal urinary concentrations of bisphenol A during pregnancy are associated with global DNA methylation in cord blood of newborns in the "NELA" birth cohort.
%D 2022
%U http://hdl.handle.net/10668/22512
%X Endocrine disrupting chemicals (EDCs) set a public health risk through disruption of normal physiological processes. The toxicoepigenetic mechanisms of developmental exposure to common EDCs, such as bisphenol A (BPA), are poorly known. The present study aimed to evaluate associations between perinatal maternal urinary concentrations of BPA, bisphenol S (BPS) and bisphenol F (BPF) and LINE-1 (long interspersed nuclear elements) and Alu (short interspersed nuclear elements, SINEs) DNA methylation levels in newborns, as surrogate markers of global DNA methylation. Data come from 318 mother-child pairs of the `Nutrition in Early Life and Asthma´ (NELA) birth cohort. Urinary bisphenol concentration was measured by dispersive liquid-liquid microextraction and ultrahigh performance liquid chromatography with tandem mass spectrometry detection. DNA methylation was quantitatively assessed by bisulphite pyrosequencing on 3 LINEs and 5 SINEs. Unadjusted linear regression analyses showed that higher concentration of maternal urinary BPA in 24th week's pregnancy was associated with an increase in LINE-1 methylation in all newborns (p = 0.01) and, particularly, in male newborns (p = 0.03). These associations remained in full adjusted models [beta = 0.09 (95 % CI = 0.03; 0.14) for all newborns; and beta = 0.10 (95 % CI = 0.03; 0.17) for males], including a non-linear association for female newborns as well (p-trend = 0.003). No associations were found between maternal concentrations of bisphenol and Alu sequences. Our results suggest that exposure to environmental levels of BPA may be associated with a modest increase in LINE-1 methylation -as a relevant marker of epigenomic stability- during human fetal development. However, any effects on global DNA methylation are likely to be small, and of uncertain biological significance.
%K Bisphenol A
%K DNA methylation
%K Endocrine disruptors
%K LINE-1
%K Perinatal exposures
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