RT Journal Article
T1 In vivo time-course biocompatibility assessment of biomagnetic nanoparticles-based biomaterials for tissue engineering applications.
A1 Campos, Fernando
A1 Bonhome-Espinosa, Ana B
A1 Carmona, Ramon
A1 Duran, Juan D G
A1 Kuzhir, Pavel
A1 Alaminos, Miguel
A1 Lopez-Lopez, Modesto T
A1 Rodriguez, Ismael A
A1 Carriel, Víctor
K1 Bio-distribution
K1 Biomaterials
K1 In vivo biocompatibility
K1 Magnetic nanoparticles
K1 Tissue engineering
AB Novel artificial tissues with potential usefulness in local-based therapies have been generated by tissue engineering using magnetic-responsive nanoparticles (MNPs). In this study, we performed a comprehensive in vivo characterization of bioengineered magnetic fibrin-agarose tissue-like biomaterials. First, in vitro analyses were performed and the cytocompatibility of MNPs was demonstrated. Then, bioartificial tissues were generated and subcutaneously implanted in Wistar rats and their biodistribution, biocompatibility and functionality were analysed at the morphological, histological, haematological and biochemical levels as compared to injected MNPs. Magnetic Resonance Image (MRI), histology and magnetometry confirmed the presence of MNPs restricted to the grafting area after 12 weeks. Histologically, we found a local initial inflammatory response that decreased with time. Structural, ultrastructural, haematological and biochemical analyses of vital organs showed absence of damage or failure. This study demonstrated that the novel magnetic tissue-like biomaterials with improved biomechanical properties fulfil the biosafety and biocompatibility requirements for future clinical use and support the use of these biomaterials as an alternative delivery route for magnetic nanoparticles.
PB Elsevier BV
SN 0928-4931
YR 2020
FD 2020-08-31
LK http://hdl.handle.net/10668/16693
UL http://hdl.handle.net/10668/16693
LA en
NO Campos F, Bonhome-Espinosa AB, Carmona R, Durán JDG, Kuzhir P, Alaminos M, et al.  In vivo time-course biocompatibility assessment of biomagnetic nanoparticles-based biomaterials for tissue engineering applications. Mater Sci Eng C Mater Biol Appl. 2021 Jan;118:111476.
NO This study was supported by grants FIS-PI17/0391 and FIS-PI17/0393 from Instituto de Salud Carlos III - ISCIII (Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica I+D+i from the Spanish Ministerio de Ciencia e Innovación), co-financed by ERDF-FEDER, European Union. Additional funding was provided by award AC17/00013 (NanoGSkin) by ISCIII through AES 2017 and within the EuroNanoMed framework, as well as grant FIS2017-85954-R from Ministerio de Economía, Industria y Competitividad (MINECO) and Agencia Estatal de Investigación (AEI), Spain, co-funded by Fondo Europeo de Desarrollo Regional (FEDER), European Union. Support was also received from grants CS PI-0257-2017 and CSyF PE-0395-2019 from Consejería de Salud y Familias, Junta de Andalucía, Spain, and grant SECYT 411/18 from SECYT (Secretary of Science and Technology of National University of Córdoba, Argentina). Further funding was provided by project Future Investments UCA JEDI (ANR-15-IDEX-01) and the project "RheoGel" by the French “Agence Nationale de la Recherche.” The authors are grateful to Dr. Ariane Ruyffelaert for her proofreading service and to Amalia de la Rosa Romero and Concepción López Rodríguez (Experimental Unit of the University Hospital Virgen de las Nieves, Granada, Spain) for their technical assistance.
DS RISalud
RD Apr 11, 2025