RT Journal Article
T1 Tracking cell proliferation using a nanotechnology-based approach.
A1 Altea-Manzano, Patricia
A1 Unciti-Broceta, Juan Diego
A1 Cano-Cortes, Victoria
A1 Ruiz-Blas, María Paz
A1 Valero-Griñan, Teresa
A1 Diaz-Mochon, Juan Jose
A1 Sanchez-Martin, Rosario
K1 cell proliferation
K1 cell tracking
K1 flow cytometry
K1 fluorescent nanoparticles
K1 nanofection
AB To develop an efficient nanotechnology fluorescence-based method to track cell proliferation to avoid the limitations of current cell-labeling dyes. Synthesis, PEGylation, bifunctionalization and labeling with a fluorophore (Cy5) of 200 nm polystyrene nanoparticles (NPs) were performed. These NPs were characterized and assessed for in vitro long-term monitoring of cell proliferation. The optimization and validation of this method to track long-term cell proliferation assays have been achieved with high reproducibility, without cell cycle disruption. This method has been successfully applied in several adherent and suspension cells including hard-to-transfect cells and isolated human primary lymphocytes. A novel approach to track efficiently cellular proliferation by flow cytometry using fluorescence labeled NPs has been successfully developed. [Formula: see text].
YR 2017
FD 2017-05-17
LK http://hdl.handle.net/10668/11202
UL http://hdl.handle.net/10668/11202
LA en
DS RISalud
RD Apr 6, 2025